Dell'Annunziata Federica, Ilisso Concetta Paola, Dell'Aversana Carmela, Greco Giuseppe, Coppola Alessandra, Martora Francesca, Dal Piaz Fabrizio, Donadio Giuliana, Falanga Annarita, Galdiero Marilena, Altucci Lucia, Galdiero Massimiliano, Porcelli Marina, Folliero Veronica, Franci Gianluigi
Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy.
Department of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy.
Microorganisms. 2020 Dec 13;8(12):1985. doi: 10.3390/microorganisms8121985.
is an opportunistic pathogen that causes nosocomial and community-acquired infections. The spread of resistant strains of represents a growing threat to human health, due to the exhaustion of effective treatments. releases outer membrane vesicles (OMVs). OMVs are a vehicle for the transport of virulence factors to host cells, causing cell injury. Previous studies have shown changes of gene expression in human bronchial epithelial cells after treatment with OMVs. These variations in gene expression could be regulated through microRNAs (miRNAs), which participate in several biological mechanisms. Thereafter, miRNA expression profiles in human bronchial epithelial cells were evaluated during infection with standard and clinical strains. Microarray analysis and RT-qPCR identified the dysregulation of miR-223, hsa-miR-21, hsa-miR-25 and hsa-let-7g miRNA sequences. Target gene prediction revealed the essential role of these miRNAs in the regulation of host immune responses involving NF-ĸB (miR-223), TLR4 (hsa-miR-21), cytokine (hsa-miR-25) and IL-6 (hsa-let-7g miRNA) signalling pathways. The current study provides the first large scale expression profile of miRNAs from lung cells and predicted gene targets, following exposure to OMVs. Our results suggest the importance of OMVs in the inflammatory response.
是一种引起医院感染和社区获得性感染的机会致病菌。由于有效治疗方法的枯竭，耐药菌株的传播对人类健康构成了日益严重的威胁。会释放外膜囊泡（OMV）。OMV是将毒力因子运输到宿主细胞的载体，会导致细胞损伤。先前的研究表明，用OMV处理后人支气管上皮细胞中的基因表达会发生变化。这些基因表达的变化可能通过参与多种生物学机制的微小RNA（miRNA）进行调节。此后，在感染标准菌株和临床菌株期间评估了人支气管上皮细胞中的miRNA表达谱。微阵列分析和RT-qPCR鉴定了miR-223、hsa-miR-21、hsa-miR-25和hsa-let-7g miRNA序列的失调。靶基因预测揭示了这些miRNA在涉及NF-κB（miR-223）、TLR4（hsa-miR-21）、细胞因子（hsa-miR-25）和IL-6（hsa-let-7g miRNA）信号通路的宿主免疫反应调节中的重要作用。本研究首次提供了暴露于OMV后肺细胞中miRNA的大规模表达谱以及预测的基因靶点。我们的结果表明OMV在炎症反应中的重要性。
