Department of Molecular Science and Technology, Ajou University, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16499, Korea.
Molecules. 2020 Dec 12;25(24):5892. doi: 10.3390/molecules25245892.
AJ003 produces glycosyl-4,4'-diaponeurosporen-4'-ol-4-oic acid as its main carotenoid. Five carotenoid pathway genes were presumed to be present in the genome of AJ003; however, 4,4-diaponeurosporene oxidase (CrtP) was non-functional, and a gene encoding aldehyde dehydrogenase (AldH) was not identified. In the present study, a genome mining approach identified two missing enzymes, CrtP2 and AldH2454, in the glycosyl-4,4'-diaponeurosporen-4'-ol-4-oic acid biosynthetic pathway. Moreover, CrtP2 and AldH enzymes were functional in heterologous and generated two carotenoid aldehydes (4,4'-diapolycopene-dial and 4,4'-diaponeurosporene-4-al) and two carotenoid carboxylic acids (4,4'-diaponeurosporenoic acid and 4,4'-diapolycopenoic acid). Furthermore, the genes encoding CrtP2 and AldH2454 were located at a distance the carotenoid gene cluster of .
AJ003 产生糖基-4,4'-二脱甲川基-4'-氧代-4'-羧酸作为其主要类胡萝卜素。假定 AJ003 的基因组中存在五个类胡萝卜素途径基因；然而，4,4-二脱甲川基叶黄素氧化酶（CrtP）无功能，并且未鉴定出编码醛脱氢酶（AldH）的基因。在本研究中，通过基因组挖掘方法在糖基-4,4'-二脱甲川基-4'-氧代-4'-羧酸生物合成途径中鉴定出两种缺失的酶，CrtP2 和 AldH2454。此外，CrtP2 和 AldH 酶在异源系统中具有功能，并产生了两种类胡萝卜素醛（4,4'-二脱甲川基-多聚copene-dial 和 4,4'-二脱甲川基-脱甲川基-4-醇）和两种类胡萝卜素羧酸（4,4'-脱甲川基-脱甲川基-4-羧酸和 4,4'-二脱甲川基-多聚 copenoic 酸）。此外，编码 CrtP2 和 AldH2454 的基因位于 的类胡萝卜素基因簇的远处。
