Perraud F, Besnard F, Sensenbrenner M, Labourdette G
Centre de Neurochimie, Strasbourg, France.
Int J Dev Neurosci. 1987;5(3):181-8. doi: 10.1016/0736-5748(87)90028-1.
Astroblasts from brain of newborn rat can survive and even proliferate to some extent in a chemically defined medium containing no other growth factor than insulin, providing they are grown first in the presence of fetal calf serum for at least 4 days (Weibel et al., 1984, Int. J. devl Neurosci. 2, 355-366). We found that thrombin is a potent mitogen for these cells, in vitro. The mitogenic activity of thrombin for astroblasts can be compared to that of the astroglial growth factor on astroblasts. However, in contrast to the bFGF, thrombin does not modify significantly the morphology of the cells and their synthesis of glutamine synthetase, an astroglial marker in rat brain. Some other proteases are also able to stimulate the proliferation of astroblasts, but to a lesser extent than thrombin. Thrombin does not stimulate the proliferation of oligodendroblasts from newborn rat and of neuroblasts from 13-day-old rat embryo. These results suggest that in the central nervous system thrombin might play a role in the induction of astrocyte proliferation after brain injury.
新生大鼠脑内的成星形细胞在一种化学限定培养基中能够存活，甚至在一定程度上增殖。该培养基除胰岛素外不含其他生长因子，前提是它们首先在胎牛血清存在的情况下培养至少4天（韦贝尔等人，1984年，《国际发育神经科学杂志》2卷，355 - 366页）。我们发现，凝血酶在体外是这些细胞的一种强效促有丝分裂原。凝血酶对成星形细胞的促有丝分裂活性可与星形胶质细胞生长因子对成星形细胞的活性相媲美。然而，与碱性成纤维细胞生长因子不同，凝血酶不会显著改变细胞形态及其谷氨酰胺合成酶的合成，谷氨酰胺合成酶是大鼠脑内星形胶质细胞的一种标志物。其他一些蛋白酶也能够刺激成星形细胞的增殖，但程度比凝血酶小。凝血酶不会刺激新生大鼠少突胶质细胞和13日龄大鼠胚胎神经母细胞的增殖。这些结果表明，在中枢神经系统中，凝血酶可能在脑损伤后星形胶质细胞增殖的诱导中起作用。
