Svoboda A, Necas O
Department of Biology, Medical Faculty, J. E. Purkynĕ University, Brno, Czechoslovakia.
J Basic Microbiol. 1987;27(10):603-12. doi: 10.1002/jobm.3620271008.
Restrictive phenotype of sec 7 mutant of Saccharomyces cerevisiae was examined by freezefracture electron microscopy. In accordance with previous findings (NOVICK et al. 1981) dictyosomes and middle-size (200-600 nm) vesicles (Berkeley bodies) were found to accumulate. Dictyosomes are formed by aggregated flattened or dilated cisternae without associated secretory vesicles. After transfer to permissive conditions the dictyosomes disappear and are not detectable, just like in the wild type or in the permissive phenotype. Associated with the restrictive phenotype is an extended plasma membrane and tonoplasts with particle-free impressions.
通过冷冻断裂电子显微镜检查酿酒酵母sec 7突变体的限制性表型。与先前的研究结果一致（诺维克等人，1981年），发现高尔基体和中等大小（200 - 600纳米）的囊泡（伯克利小体）积累。高尔基体由聚集的扁平或扩张的潴泡形成，没有相关的分泌囊泡。转移到允许条件下后，高尔基体消失且无法检测到，就像在野生型或允许表型中一样。与限制性表型相关的是扩展的质膜和无颗粒印记的液泡膜。
