Sidorskii Egor V, Krasnov Mikhail S, Yamskova Viktoria P, Lozinsky Vladimir I
A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street, 28, 119991 Moscow, Russia.
Institute of Bioregulation Problems, Leninskii Avenue 45, 119334 Moscow, Russia.
Gels. 2020 Dec 14;6(4):50. doi: 10.3390/gels6040050.
Wide-pore proteinaceous freeze-thaw spongy gels were synthesized via the cryotropic gelation technique using the bovine blood serum or its diluted solutions as the protein-containing precursors. The feed systems also included the denaturant (urea) and the thiol-reductant (cysteine). The gel-fraction yield decreased and the swelling degree of the walls of macropores in such heterophase matrices increased with decreasing the initial protein concentration. The optimum freezing temperature was found to be within a rather narrow range from -15 to -20 °C. In this case, the average size of the macropores in the resultant cryogels was 90-110 μm. The suitability of such soft wide-pore gel materials for the application as the carriers of peptide bioregulators was demonstrated in the in vitro experiments, when the posterior segments of the adult newts' eyes were used as a model biological target. It was shown that a statistically reliable protective effect on the state of the sclera, vascular membrane and retinal pigment epithelium, as well as on the viability of fibroblasts, was inherent in the proteinaceous cryogels loaded with the peptide bioregulator (Viophtan-5™) isolated from the bovine eye sclera.
通过低温凝胶化技术，以牛血清或其稀释溶液作为含蛋白质的前体，合成了宽孔蛋白质冻融海绵凝胶。进料系统还包括变性剂（尿素）和硫醇还原剂（半胱氨酸）。随着初始蛋白质浓度的降低，这种异相基质中的凝胶分数产率降低，大孔壁的溶胀度增加。发现最佳冷冻温度在-15至-20°C的相当窄的范围内。在这种情况下，所得冷冻凝胶中大孔的平均尺寸为90-110μm。当将成年蝾螈眼睛的后段用作模型生物靶标时，在体外实验中证明了这种柔软的宽孔凝胶材料作为肽生物调节剂载体应用的适用性。结果表明，负载从牛眼巩膜分离的肽生物调节剂（Viophtan-5™）的蛋白质冷冻凝胶对巩膜、血管膜和视网膜色素上皮的状态以及成纤维细胞的活力具有统计学上可靠的保护作用。
