Yan Huimin, Sun Hongzheng, Jia Xueying, Lv Chuanwei, Li Junzhou, Zhao Quanzhi
Henan Key Laboratory of Rice Biology, Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, China.
Front Plant Sci. 2020 Nov 23;11:575304. doi: 10.3389/fpls.2020.575304. eCollection 2020.
Cytokinins are crucial signaling molecules that regulate plant growth and development. irreversibly degrades nucleobase cytokinins by encoding cytokinin oxidase/dehydrogenase to control grain production in rice. In this study, was specifically overexpressed in roots using promoter to investigate the effects of root-source cytokinins on the growth of rice. overexpressed (OE) rice showed retarded growth with lower cytokinin levels and biomass production. Shoot-specific transcriptome analysis between OE rice and wild type (WT) revealed differentially expressed genes (DEGs) associated with cell division, cell wall structure, phytohormone signaling, and assimilation and catabolism. Metabolome analysis indicated that a majority of differential primary metabolites, such as amino acids and organic acids, increased, while lipids decreased in OE rice. Integration of transcriptomic and metabolomic data showed that several DEGs and differential metabolites were related to glycolysis and tricarboxylic acid cycle (TCA). To conclude, reduced cytokinin levels via root-specific overexpression of resulted in developmental defects, which confirmed the importance of root-source cytokinins in plant growth and morphogenesis.
细胞分裂素是调节植物生长发育的关键信号分子。通过编码细胞分裂素氧化酶/脱氢酶不可逆地降解核苷碱基细胞分裂素，以控制水稻的籽粒产量。在本研究中，使用启动子在根部特异性过表达，以研究根源细胞分裂素对水稻生长的影响。过表达（OE）水稻生长受阻，细胞分裂素水平和生物量产量较低。对OE水稻和野生型（WT）进行的地上部特异性转录组分析揭示了与细胞分裂、细胞壁结构、植物激素信号传导以及同化和分解代谢相关的差异表达基因（DEG）。代谢组分析表明，OE水稻中大多数差异初级代谢产物，如氨基酸和有机酸增加，而脂质减少。转录组和代谢组数据的整合表明，一些DEG和差异代谢产物与糖酵解和三羧酸循环（TCA）有关。总之，通过根部特异性过表达降低细胞分裂素水平导致发育缺陷，这证实了根源细胞分裂素在植物生长和形态发生中的重要性。
