Mukushkina Dina, Aisina Dana, Pyrkova Anna, Ryskulova Alma, Labeit Siegfried, Ivashchenko Anatoliy
Department of Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
Department of microbiology, Kazakh Medical University of Continuing Education, Almaty, Kazakhstan.
Front Genet. 2020 Nov 4;11:605054. doi: 10.3389/fgene.2020.605054. eCollection 2020.
The involvement of genes and miRNAs in the development of atherosclerosis is a challenging problem discussed in recent publications. It is necessary to establish which miRNAs affect the expression of candidate genes. We used known candidate atherosclerosis genes to predict associations. The quantitative characteristics of interactions of miRNAs with mRNA candidate genes were determined using the program, which identifies the localization of miRNA binding sites in mRNA, the free energy interaction of miRNA with mRNA. In mRNAs of and candidate genes, binding sites of 21 miRNAs and of 15 miRNAs, respectively, were identified. In mRNA binding sites of 25 miRNAs were located in a cluster of 41 nt. In , and mRNAs, clusters of miR-466, ID00436.3p-miR, and ID01030.3p-miR BS were identified. The organization of overlapping miRNA binding sites in clusters led to their compaction and caused competition among the miRNAs. The binding of 53 miRNAs to the mRNAs of 14 candidate genes with free energy interactions greater than -130 kJ/mole was determined. The miR-619-5p was fully complementary to and mRNAs, ID01593.5p-miR to mRNA, ID01935.5p-miR to , and miR-5096 to mRNA. Associations of miRNAs and candidate atherosclerosis genes are proposed for the early diagnosis of this disease.
基因和微小RNA（miRNA）在动脉粥样硬化发展过程中的作用是近期出版物中讨论的一个具有挑战性的问题。确定哪些miRNA影响候选基因的表达很有必要。我们使用已知的动脉粥样硬化候选基因来预测关联。利用该程序确定了miRNA与mRNA候选基因相互作用的定量特征，该程序可识别miRNA在mRNA中的结合位点定位以及miRNA与mRNA的自由能相互作用。在[具体基因1]和[具体基因2]的mRNA中，分别鉴定出了21个miRNA和15个miRNA的结合位点。在[具体基因3]的mRNA中，25个miRNA的结合位点位于一个41个核苷酸的簇中。在[具体基因4]、[具体基因5]和[具体基因6]的mRNA中，鉴定出了miR - 466、ID00436.3p - miR和ID01030.3p - miR BS的簇。簇中重叠miRNA结合位点的组织导致了它们的紧密排列，并引起了miRNA之间的竞争。确定了53个miRNA与14个候选基因的mRNA的结合，其自由能相互作用大于 - 130 kJ/摩尔。miR - 619 - 5p与[具体基因7]和[具体基因8]的mRNA完全互补，ID01593.5p - miR与[具体基因9]的mRNA完全互补，ID01935.5p - miR与[具体基因10]的mRNA完全互补，miR - 5096与[具体基因11]的mRNA完全互补。提出了miRNA与动脉粥样硬化候选基因的关联，用于该疾病的早期诊断。
