微小 RNA-183-5p 可作为动脉粥样硬化的潜在诊断生物标志物，并调节血管平滑肌细胞的生长。
Micro-RNA-183-5p acts as a potential diagnostic biomarker for atherosclerosis and regulates the growth of vascular smooth muscle cell.
机构信息
Department of Emergency, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
Department of Critical Care Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
出版信息
J Chin Med Assoc. 2021 Jan 1;84(1):33-37. doi: 10.1097/JCMA.0000000000000433.
BACKGROUND
Atherosclerosis (AS) is a multifactorial chronic disease, and vascular smooth muscle cells (VSMCs) play important roles in the pathology of AS. Micro-RNAs regulate multiple cellular biological processes. This study aimed to investigate the clinical value of miR-183-5p in AS patients and further explored the effects of miR-183-5p on the proliferation and migration of VSMCs.
METHODS
Quantitative real-time polymerase chain reaction was used to test the level of miR-183-5p. The diagnostic value of miR-183-5p for AS patients was assessed by a receiver operating characteristic (ROC) analysis. The proliferation and migration of VSMCs were determined via the CCK-8 and Transwell assay.
RESULTS
MiR-183-5p was highly expressed in AS patients compared with the healthy group. Serum miR-183-5p expression was positively associated with carotid intima-media thickness and C-reactive protein in AS patients. The ROC analysis suggested that miR-183-5p had the quality to be used as a biomarker with high specificity and sensitivity for AS detection. Overexpression of miR-183-5p promoted the proliferation and migration of VSMCs.
CONCLUSION
MiR-183-5p is highly expressed in AS patients, which promotes the proliferation and migration of VSMCs. MiR-183-5p may be a key molecular in the diagnosis and treatment of AS in the future.
背景
动脉粥样硬化（AS）是一种多因素的慢性疾病，血管平滑肌细胞（VSMCs）在 AS 的病理中起着重要作用。微小 RNA 调节多种细胞的生物学过程。本研究旨在探讨 miR-183-5p 在 AS 患者中的临床价值，并进一步探讨 miR-183-5p 对 VSMCs 增殖和迁移的影响。
方法
采用实时定量聚合酶链反应检测 miR-183-5p 的水平。通过受试者工作特征（ROC）分析评估 miR-183-5p 对 AS 患者的诊断价值。通过 CCK-8 和 Transwell 测定检测 VSMCs 的增殖和迁移。
结果
与健康组相比，AS 患者中 miR-183-5p 表达水平较高。AS 患者血清 miR-183-5p 表达与颈动脉内膜中层厚度和 C-反应蛋白呈正相关。ROC 分析表明，miR-183-5p 具有作为 AS 检测高特异性和高灵敏度生物标志物的潜力。miR-183-5p 的过表达促进了 VSMCs 的增殖和迁移。
结论
miR-183-5p 在 AS 患者中表达水平较高，促进了 VSMCs 的增殖和迁移。miR-183-5p 可能是未来 AS 诊断和治疗的关键分子。
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