Department of Emergency, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
Department of Critical Care Medicine, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
J Chin Med Assoc. 2021 Jan 1;84(1):33-37. doi: 10.1097/JCMA.0000000000000433.
Atherosclerosis (AS) is a multifactorial chronic disease, and vascular smooth muscle cells (VSMCs) play important roles in the pathology of AS. Micro-RNAs regulate multiple cellular biological processes. This study aimed to investigate the clinical value of miR-183-5p in AS patients and further explored the effects of miR-183-5p on the proliferation and migration of VSMCs.
Quantitative real-time polymerase chain reaction was used to test the level of miR-183-5p. The diagnostic value of miR-183-5p for AS patients was assessed by a receiver operating characteristic (ROC) analysis. The proliferation and migration of VSMCs were determined via the CCK-8 and Transwell assay.
MiR-183-5p was highly expressed in AS patients compared with the healthy group. Serum miR-183-5p expression was positively associated with carotid intima-media thickness and C-reactive protein in AS patients. The ROC analysis suggested that miR-183-5p had the quality to be used as a biomarker with high specificity and sensitivity for AS detection. Overexpression of miR-183-5p promoted the proliferation and migration of VSMCs.
MiR-183-5p is highly expressed in AS patients, which promotes the proliferation and migration of VSMCs. MiR-183-5p may be a key molecular in the diagnosis and treatment of AS in the future.
动脉粥样硬化（AS）是一种多因素的慢性疾病，血管平滑肌细胞（VSMCs）在 AS 的病理中起着重要作用。微小 RNA 调节多种细胞的生物学过程。本研究旨在探讨 miR-183-5p 在 AS 患者中的临床价值，并进一步探讨 miR-183-5p 对 VSMCs 增殖和迁移的影响。
采用实时定量聚合酶链反应检测 miR-183-5p 的水平。通过受试者工作特征（ROC）分析评估 miR-183-5p 对 AS 患者的诊断价值。通过 CCK-8 和 Transwell 测定检测 VSMCs 的增殖和迁移。
与健康组相比，AS 患者中 miR-183-5p 表达水平较高。AS 患者血清 miR-183-5p 表达与颈动脉内膜中层厚度和 C-反应蛋白呈正相关。ROC 分析表明，miR-183-5p 具有作为 AS 检测高特异性和高灵敏度生物标志物的潜力。miR-183-5p 的过表达促进了 VSMCs 的增殖和迁移。
miR-183-5p 在 AS 患者中表达水平较高，促进了 VSMCs 的增殖和迁移。miR-183-5p 可能是未来 AS 诊断和治疗的关键分子。
