Kim Min-Ho, Baek Ki-Ook, Park Gyeong-Gook, Jang Je-Youn, Lee Jin-Hong
Department of Work Environment Monitoring, Armed Forces Medical Research Institute, 90, Jaun-ro, Yuseong-gu, Daejeon, Republic of Korea.
Department of Environmental Engineering, Chungnam National University of Engineering, 99, Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
Saf Health Work. 2020 Dec;11(4):517-525. doi: 10.1016/j.shaw.2020.09.002. Epub 2020 Sep 8.
The study was planned to show the status of indoor microorganisms and the status of the reduction device in the military dog clinic.
Airborne microbes were analyzed according to the number of daily patient canines. For identification of bacteria, sampled bacteria was identified using VITEK®2 and molecular method. The status of indoor microorganisms according to the operation of the ventilation system was analyzed.
Airborne bacteria and fungi concentrations were 1000.6 ± 800.7 CFU/m and 324.7 ± 245.8 CFU/m. In the analysis using automated identification system, based on fluorescence biochemical test, VITEK®2, mainly human pathogenic bacteria were identified. The three most frequently isolated genera were (26.6%), (24.48%), and (12.7%). The results analyzed by molecular method were detected in the order of (22.6%), followed by (18.1%), (11.1%), and so on. When the ventilation system was operated appropriately, the airborne bacteria and fungi level were significantly decreased.
Airborne bacteria in the clinic tend to increase with the number of canines. Human pathogenic bacteria were mainly detected in VITEK®2, and relatively various bacteria were detected in molecular analysis. A decrease in the level of bacteria and fungi was observed with proper operation of the ventilation system.
本研究旨在展示军犬诊所室内微生物的状况以及净化装置的状况。
根据每日就诊犬只数量对空气传播微生物进行分析。对于细菌鉴定，使用VITEK®2和分子方法对采样细菌进行鉴定。分析了通风系统运行情况下室内微生物的状况。
空气传播细菌和真菌的浓度分别为1000.6 ± 800.7 CFU/m和324.7 ± 245.8 CFU/m。在使用基于荧光生化试验的自动鉴定系统VITEK®2进行的分析中，主要鉴定出人类病原菌。最常分离出的三个属依次为（26.6%）、（24.48%）和（12.7%）。分子方法分析的结果按顺序检测到（22.6%），其次是（18.1%）、（11.1%）等。当通风系统正常运行时，空气传播细菌和真菌水平显著降低。
诊所内空气传播细菌往往随着犬只数量的增加而增多。在VITEK®2检测中主要检测到人类病原菌，分子分析中检测到相对多样的细菌。通风系统正常运行时，观察到细菌和真菌水平下降。
