Huang Qingting, Sun Yun, Wang Weiwei, Lin Lien-Chun, Huang Yangle, Yang Jing, Wu Xiaodong, Kong Lin, Lu Jiade Jay
Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Shanghai, China.
Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, China.
Front Oncol. 2020 Nov 19;10:597702. doi: 10.3389/fonc.2020.597702. eCollection 2020.
Recently, a growing number of studies focus on partial tumor irradiation to induce the stronger non-target effects. However, the value of partial volume carbon ion radiotherapy (CIRT) targeting hypoxic region of a tumor under imaging guidance as well as its effect of inducing radiation induced abscopal effects (RIAEs) have not been well investigated. Herein, we developed a technique of carbon ion microporous radiation (CI-MPR), guided by F-FMISO PET/computerized tomography (CT), for partial volume radiation targeting the hypoxia area of a tumor and investigated its capability of inducing abscopal effects. Tumor-bearing mice were inoculated subcutaneously with breast cancer 4T1 cells into the flanks of both hind legs of mouse. Mice were assigned to three groups: group I: control group with no treatment; group II: carbon ion open field radiation (CI-OFR group) targeting the entire tumor; group III: partial volume carbon ion microporous radiation (CI-MPR group) targeting the hypoxia region. The tumors on the left hind legs of mice were irradiated with single fraction of 20 Gy of CIRT. Mice treated with CI-MPR or CI-OFR showed that significant growth delay on both the irradiated and unirradiated of tumor as compared to the control groups. Tumor regression of left tumor irradiated with CI-OFR was more prominent as compared to the tumor treated with CI-MPR, while the regression of the unirradiated tumor in both CI-MPR and CI-OFR group was similar. Biological-guided CIRT using the newly developed microporous technique targeting tumor hypoxia region could induce robust abscopal effects similar to CIRT covering the entire tumor.
最近，越来越多的研究聚焦于局部肿瘤照射以诱导更强的非靶效应。然而，在成像引导下针对肿瘤缺氧区域的局部容积碳离子放疗（CIRT）的价值及其诱导放射诱导远隔效应（RIAEs）的效果尚未得到充分研究。在此，我们开发了一种由F-FMISO正电子发射断层显像/计算机断层扫描（PET/CT）引导的碳离子微孔放射（CI-MPR）技术，用于针对肿瘤缺氧区域的局部容积放射，并研究其诱导远隔效应的能力。将荷瘤小鼠双侧后肢腹股沟皮下接种乳腺癌4T1细胞。小鼠分为三组：第一组：未治疗的对照组；第二组：针对整个肿瘤的碳离子开放野放射（CI-OFR组）；第三组：针对缺氧区域的局部容积碳离子微孔放射（CI-MPR组）。对小鼠左后肢的肿瘤进行单次20 Gy的碳离子放疗。与对照组相比，接受CI-MPR或CI-OFR治疗的小鼠在照射和未照射的肿瘤上均显示出显著的生长延迟。与接受CI-MPR治疗的肿瘤相比，接受CI-OFR照射的左肿瘤的消退更为显著，而CI-MPR组和CI-OFR组中未照射肿瘤的消退情况相似。使用新开发的针对肿瘤缺氧区域的微孔技术进行生物引导的CIRT可诱导与覆盖整个肿瘤的CIRT相似的强大远隔效应。
