Division of Infection Medicine, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
Department of Infection Medicine, Skåne University Hospital, Lund, Sweden.
Front Cell Infect Microbiol. 2020 Nov 20;10:571578. doi: 10.3389/fcimb.2020.571578. eCollection 2020.
is a common infectious agent in sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of . Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence ( anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (K = 2.75 x 10 M) and demonstrated a statistically significant difference ( < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.
是脓毒症中的一种常见感染因子，与高死亡率和严重的长期影响有关。细胞溶蛋白α-溶血素已被反复证明可增强的毒力。再加上多药耐药株不受阻碍的传播，这引发了对新型抗毒力（抗α-溶血素）药物的研究。它们的功能将取决于我们识别可能通过这种方式缓解的感染的能力。因此，我们需要能够识别α-溶血素是主要决定因素的感染患者的检测方法。随后在金涂覆的传感器芯片上制备了分子印迹聚合物。与表面等离子体共振生物传感器结合使用，可从脓毒症血液样本（n = 9）中定量α-溶血素，而无需对感染因子进行预培养。该生物传感器对α-溶血素有高亲和力（K = 2.75 x 10 M），并且在α-溶血素反应与潜在样品污染物之间表现出统计学上的显著差异（<0.0001）。与基于抗体的检测方法相比，该检测方案同样出色，甚至更好。与许多当前使用的方法相比，这种新型检测方案构成了一种更快速、经济且用户友好的替代方案。分子印迹与表面等离子体共振（SPR）技术的结合提高了重现性和灵敏度，可能成为临床和研究环境中的一种多功能新工具。
