Molecular Biology and Biochemistry Laboratory, Institute of Biological Sciences II, Federal University of Goias (UFG), Goiania, Brazil.
Laboratory of Experimental Microbiology, State University of Western Paraná (Unioeste), Cascavel, Brazil.
Front Cell Infect Microbiol. 2020 Nov 30;10:573097. doi: 10.3389/fcimb.2020.573097. eCollection 2020.
is a thermodimorphic fungus that causes histoplasmosis, a mycosis of global incidence. The disease is prevalent in temperate and tropical regions such as North America, South America, Europe, and Asia. It is known that during infection macrophages restrict Zn availability to as a microbicidal mechanism. In this way the present work aimed to study the response of to zinc deprivation. analyses showed that has eight genes related to zinc homeostasis ranging from transcription factors to CDF and ZIP family transporters. The transcriptional levels of , , and were induced under zinc-limiting conditions. The decrease in Zn availability increases fungicidal macrophage activity. Proteomics analysis during zinc deprivation at 24 and 48 h showed 265 proteins differentially expressed at 24 h and 68 at 48 h. Proteins related to energy production pathways, oxidative stress, and cell wall remodeling were regulated. The data also suggested that low metal availability increases the chitin and glycan content in fungal cell wall that results in smoother cell surface. Metal restriction also induces oxidative stress triggered, at least in part, by reduction in pyridoxin synthesis.
组织胞浆菌是一种温度双相真菌，可引起组织胞浆菌病，这是一种具有全球发病率的真菌病。该疾病常见于中纬度和热带地区，如北美洲、南美洲、欧洲和亚洲。已知在感染期间，巨噬细胞会限制锌的可用性，以此作为一种杀菌机制。因此，本研究旨在研究 对锌剥夺的反应。分析表明，组织胞浆菌有八个与锌稳态相关的基因，范围从转录因子到 CDF 和 ZIP 家族转运蛋白。在缺锌条件下， 、 和 的转录水平被诱导。锌可用性的降低会增加杀真菌性巨噬细胞的活性。在 24 和 48 小时锌剥夺期间的蛋白质组学分析显示，24 小时时有 265 种蛋白质差异表达，48 小时时有 68 种蛋白质差异表达。与能量产生途径、氧化应激和细胞壁重塑相关的蛋白质受到调控。数据还表明，低金属可用性会增加真菌细胞壁中的几丁质和聚糖含量，从而使细胞表面更加光滑。金属限制还会引发氧化应激，至少部分是由吡哆醇合成减少引起的。
