[具体对象]膜蛋白质组的鉴定及其锌调控
Identification of membrane proteome of and its regulation by zinc.
作者信息
de Curcio Juliana Santana, Silva Marielle Garcia, Silva Bailão Mirelle Garcia, Báo Sônia Nair, Casaletti Luciana, Bailão Alexandre Mello, de Almeida Soares Célia Maria
机构信息
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Laboratório de Microscopia, Universidade de Brasília, Brasília, Brazil.
出版信息
Future Sci OA. 2017 Jul 25;3(4):FSO232. doi: 10.4155/fsoa-2017-0044. eCollection 2017 Nov.
AIM
During infection development in the host, spp. faces the deprivation of micronutrients, a mechanism called nutritional immunity. This condition induces the remodeling of proteins present in different metabolic pathways. Therefore, we attempted to identify membrane proteins and their regulation by zinc in .
MATERIALS & METHODS: Membranes enriched fraction of yeast cells of were isolated, purified and identified by 2D LC-MS/MS detection and database search.
RESULTS & CONCLUSION: Zinc deprivation suppressed the expression of membrane proteins such as glycoproteins, those involved in cell wall synthesis and those related to oxidative phosphorylation. This is the first study describing membrane proteins and the effect of zinc deficiency in their regulation in one member of the genus .
目的
在宿主体内感染发展过程中，[物种名称]面临微量营养素缺乏的情况，这一机制被称为营养免疫。这种状况会诱导不同代谢途径中存在的蛋白质发生重塑。因此，我们试图鉴定[物种名称]中的膜蛋白及其受锌的调控情况。
材料与方法
分离、纯化了[物种名称]酵母细胞的富含膜的部分，并通过二维液相色谱-串联质谱检测和数据库搜索进行鉴定。
结果与结论
锌缺乏抑制了膜蛋白的表达，如糖蛋白、参与细胞壁合成的蛋白以及与氧化磷酸化相关的蛋白。这是第一项描述[物种名称]属中一个成员的膜蛋白及其锌缺乏对其调控影响的研究。
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