Yoon Jeong Yong, Lee Seung Yeon, Shin Sue, Yoon Kang Sup, Jo Chris Hyunchul
Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
Department of Laboratory Medicine, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea.
Clin Shoulder Elb. 2018 Mar 1;21(1):3-14. doi: 10.5397/cise.2018.21.1.3. eCollection 2018 Mar.
Platelet-rich plasma (PRP) stimulates cell proliferation and enhances matrix gene expression and synthesis. However, there have been no comparative study of the PRP effect on the normal and degenerative tenocytes. The purpose of this study was to compare the effect of PRP on tenocytes from normal and degenerative tendon.
Tendon tissues were obtained from patients undergoing arthroscopic repair (n=9) and from healthy donors (n=3). Tenocytes were cultured with 10% (vol/vol) platelet-poor plasma, PRP activated with calcium, and PRP activated with calcium and thrombin. The total cell number was assessed at days 7 and 14. The expressions of type I and III collagen, decorin, tenascin-C, and scleraxis were evaluated by quantitative real-time reverse transcriptase polymerase chain reaction. The total collagen and glycosaminoglycan (GAG) synthesis was evaluated at days 7 and 14.
No differences were observed between the groups at day 7, but cell proliferation was remarkably increased in tenocytes from the degenerative tendon at day 14. In both tenocyte groups, the gene expressions of type I and III collagen were up-regulated. GAG synthesis was greater in the normal tendon, whereas the expressions of decorin and tenascin-C were increased in tenocytes from the degenerative tendon. Tenocytes from the degenerative tendon had higher fold-change of GAG synthesis and a lower collagen III/I ratio than normal tenocytes.
PRP promoted the cell proliferation and enhanced the synthesis of tendon matrix in both groups. PRP has a greater positive effect on cell proliferation, matrix gene expression and synthesis in tenocytes from degenerative tendon.
富血小板血浆（PRP）可刺激细胞增殖，并增强基质基因表达与合成。然而，尚无关于PRP对正常和退变肌腱细胞作用的比较研究。本研究旨在比较PRP对正常和退变肌腱中肌腱细胞的影响。
从接受关节镜修复的患者（n = 9）和健康供体（n = 3）获取肌腱组织。将肌腱细胞分别与10%（体积/体积）的贫血小板血浆、钙激活的PRP以及钙和凝血酶激活的PRP共同培养。在第7天和第14天评估细胞总数。通过定量实时逆转录聚合酶链反应评估I型和III型胶原蛋白、核心蛋白聚糖、肌腱蛋白-C和硬骨素的表达。在第7天和第14天评估总胶原蛋白和糖胺聚糖（GAG）的合成。
第7天时各组之间未观察到差异，但在第14天时，退变肌腱中的肌腱细胞增殖显著增加。在两个肌腱细胞组中，I型和III型胶原蛋白的基因表达均上调。正常肌腱中的GAG合成更多，而退变肌腱中的肌腱细胞中核心蛋白聚糖和肌腱蛋白-C的表达增加。退变肌腱中的肌腱细胞比正常肌腱细胞具有更高的GAG合成倍数变化和更低的胶原蛋白III/I比值。
PRP促进了两组中的细胞增殖并增强了肌腱基质的合成。PRP对退变肌腱中的肌腱细胞的细胞增殖、基质基因表达和合成具有更大的积极作用。
