School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, India.
Lett Appl Microbiol. 2021 May;72(5):517-525. doi: 10.1111/lam.13441. Epub 2021 Jan 11.
Bacteria survive metal stress by several mechanisms and metal binding is one such mechanism which has been screened in the present study to investigate the survival strategies of metal resistant bacteria. The production of siderophores, a metal chelating agent, was detected by chrome azurol S agar assay. The changes in cell wall studied by analysing the peptidoglycan and teichoic acid content indicated an increase in the cell wall content. Evaluation of morphological and physiological alterations like cell size, granularity analysed by SEM and flow cytometry analysis revealed an increase in cell size and granularity respectively. The transformation of phosphates monitored by P NMR analysis indicated the presence of inorganic phosphate. Based on the cell wall changes and the P NMR analysis, the surface charge of the organism was studied by zeta potential which displayed a difference at pH7.
细菌通过多种机制来应对金属胁迫，金属结合就是其中一种机制，本研究通过筛选来检测金属抗性细菌的生存策略。通过显色法检测到细菌产生了铁载体，这是一种金属螯合剂。通过分析肽聚糖和磷壁酸的含量来研究细胞壁的变化，结果表明细胞壁的含量增加了。通过 SEM 和流式细胞术分析来评估细胞大小、颗粒度等形态和生理变化，结果表明细胞大小和颗粒度都增加了。通过 31P NMR 分析监测磷酸盐的转化，结果表明存在无机磷酸盐。基于细胞壁的变化和 31P NMR 分析，通过zeta 电位研究了生物体的表面电荷，结果表明在 pH7 时存在差异。
