Faculty of Engineering, University of Yamanashi, 4-3-11, Takeda, Kofu, Yamanashi 400-8511, Japan.
Department of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi, 1110, Shimokato, Chuo, Yamanashi 409-3898, Japan.
J Am Soc Mass Spectrom. 2021 Feb 3;32(2):606-610. doi: 10.1021/jasms.0c00366. Epub 2020 Dec 17.
A moving string sampling probe and a new ESI based ionization source that can be readily incorporated into the existing endoscopes are developed for performing mass spectrometry during the endoscopic procedure. The medical-grade silk suture driven by a stepping motor is used to perform the sampling on the region of interest when the probe head is brought gently into contact with the surface of the gastrointestinal tissue. The tissues and the compounds adhered to the sampling string are transported to an ionization region inside the ion inlet tube in which they are extracted and ionized by the charging droplets generated from an electrospray outside the ion inlet. Since the extraction/ionization and sampling processes are isolated, organic solvents, high voltage (HV), and heating can be used for the optimization of ionization without compromising the biocompatibility of the sampling probe. The demonstration of the analysis of the gastric mucosa of a mouse is performed using a 2 m long gastrointestinal endoscope.
为了在内窥镜手术过程中进行质谱分析，我们开发了一种可移动的弦线采样探头和一种新的基于 ESI 的可轻易集成到现有内窥镜的离子源。当探头轻轻接触胃肠道组织表面时，由步进电机驱动的医用级丝线缝合线用于对感兴趣区域进行采样。附着在采样线上的组织和化合物被输送到离子入口管内的离子化区域，在那里，它们通过从离子入口外的电喷雾产生的荷电液滴被提取和离子化。由于提取/离子化和采样过程是隔离的，因此可以使用有机溶剂、高压 (HV) 和加热来优化离子化，而不会损害采样探头的生物相容性。我们使用 2 米长的胃肠道内窥镜演示了对小鼠胃黏膜的分析。
