Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11, Takeda, Kofu, Yamanashi, 400-8511 Japan.
Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Japan.
Analyst. 2017 Jul 24;142(15):2735-2740. doi: 10.1039/c7an00650k.
At present, endoscopy relies almost exclusively on optical microscopy and the accurate analysis such as MS interrogation is performed ex situ using biopsy. In this work, a novel probing system is developed to perform in situ and in vivo endoscopic mass spectrometry using a moving string for the sampling and transportation of material. A prototype of a mass spectrometric endoscope is constructed using an industrial endoscope and a commercial mass spectrometer. The sampling system consists of a moving cotton thread driven by motorized pulleys. When the target surface is touched by the sampling probe, the cotton thread "wipes" and transports the adhered sample to the ion source. Depending on the target analytes, desorption electrospray and atmospheric pressure chemical ionization sources are employed interchangeably for the desorption and ionization. The surface under analysis is not subjected to heat, organic solvents, high voltage or charged droplets. In situ endoscopic MS of a living mouse and surface analysis inside a volunteer subject's mouth are demonstrated.
目前，内窥镜几乎完全依赖光学显微镜，并且使用活检进行 MS 询问等精确分析都是在体外进行的。在这项工作中，开发了一种新的探测系统，使用移动线绳在体内和体内进行内窥镜质谱分析，用于采样和输送材料。使用工业内窥镜和商用质谱仪构建了质谱内窥镜的原型。采样系统由电动滑轮驱动的移动棉线组成。当采样探针接触到目标表面时，棉线“擦拭”并将附着的样品输送到离子源。根据目标分析物，可交替使用解吸电喷雾和大气压化学电离源进行解吸和电离。待分析的表面不会受到热、有机溶剂、高压或带电液滴的影响。展示了对活体小鼠进行的体内内窥镜 MS 以及对志愿者口腔内部的表面分析。
