Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, University of California, Irvine, CA 92697-4625, USA.
Lab Chip. 2021 Jan 21;21(2):405-411. doi: 10.1039/d0lc00970a. Epub 2020 Dec 17.
A novel lab-in-a-pencil graphite microfluidic sensing electrode (μFSE) was fabricated for real-time flow injection measurement of the antipsychotic drug clozapine (Clz). A simple, low-cost, and reusable μFSE was obtained by using 3D printing of a microfluidic chamber integrated with a flat pencil graphite without the need to utilize complex technologies. The μFSE has tubular geometry with 800 μm diameter, where the solution continuously flows in the holes of flat pencil graphite electrodes. Under optimized conditions, this device offers fast and effective Clz detection with good analytical features. A linear calibration curve in the range of 0.5 to 10 μM Clz was obtained with good sensitivity (0.01275 μA μM) and detection limit (24 nM). Finally, we demonstrate the applicability of our lab-fabricated microfluidic electrochemical device by monitoring Clz in serum samples at low concentrations.
一种新型的笔式实验室石墨微流控传感电极（μFSE）被用于实时流动注射测量抗精神病药物氯氮平（Clz）。通过使用集成有平面铅笔石墨的微流控室的 3D 打印，获得了一种简单、低成本和可重复使用的μFSE，而无需使用复杂的技术。μFSE 具有 800μm 直径的管状几何形状，其中溶液在平面铅笔石墨电极的孔中连续流动。在优化条件下，该装置具有快速有效的 Clz 检测能力，具有良好的分析特性。在 0.5 至 10μM Clz 的范围内获得了线性校准曲线，具有良好的灵敏度（0.01275μAμM）和检测限（24nM）。最后，我们通过监测低浓度血清样品中的 Clz，证明了我们实验室制造的微流控电化学装置的适用性。
