Hiroshima University, Graduate School of Biomedical and Health Sciences, Program of Oral Health Sciences, Department of Public Oral Health, Hiroshima, Japan.
Hiroshima University, Graduate School of Biomedical and Health Sciences, Program of Oral Health Sciences, Department of Oral Health Management, Hiroshima, Japan.
J Appl Oral Sci. 2020 Dec 18;28:e20200501. doi: 10.1590/1678-7757-2020-00501. eCollection 2020.
This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults.
In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples were taken to examine the presence of HCMV DNA using real-time polymerase chain reaction (PCR). P. gingivalis was detected by semi-quantitative PCR analysis.
HCMV DNA was present in nine of 190 patients (4.7%). There were significant associations between HCMV presence and the presence of ≥4-mm-deep periodontal pockets with bleeding on probing (BOP) (P<0.01) and ≥6-mm-deep periodontal pockets with BOP (P=0.01). However, no significant relationship was observed between HCMV presence and periodontal epithelial surface area scores. Logistic regression analysis revealed that the presence of ≥4-mm-deep periodontal pockets with BOP was significantly associated with HCMV (odds ratio, 14.4; P=0.01). Propensity score matching was performed between patients presenting ≥4-mm-deep periodontal pockets with BOP (i.e., active periodontitis) and patients without ≥4-mm-deep periodontal pockets with BOP; 62 matched pairs were generated. Patients who had ≥4-mm-deep periodontal pockets with BOP showed a higher rate of HCMV presence (9.7%) than those who lacked ≥4-mm-deep periodontal pockets with BOP (0.0%). There was a significant relationship between HCMV presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). A significant relationship was found between HCMV/P. gingivalis DNA presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03).
Coinfection of oral HCMV and P. gingivalis was significantly associated with active periodontitis. Moreover, interactions between oral HCMV and P. gingivalis may be related to the severity of periodontal disease.
本研究旨在阐明日本成年人中口腔人巨细胞病毒（HCMV）与牙周炎之间的关联。
共纳入 190 例患者（男 75 例，女 115 例；平均年龄 70.2 岁），均于 2018 年 3 月至 2020 年 5 月期间在广岛大学医院就诊。采用实时聚合酶链反应（PCR）检测口腔冲洗样本中 HCMV DNA 的存在情况。采用半定量 PCR 分析检测牙龈卟啉单胞菌。
190 例患者中，有 9 例（4.7%）存在 HCMV DNA。HCMV 存在与探诊出血的≥4mm 深牙周袋（P<0.01）和探诊出血的≥6mm 深牙周袋（P=0.01）显著相关。然而，HCMV 存在与牙周上皮表面面积评分之间无显著关系。Logistic 回归分析显示，探诊出血的≥4mm 深牙周袋的存在与 HCMV 显著相关（比值比，14.4；P=0.01）。在探诊出血的≥4mm 深牙周袋（即活动性牙周炎）患者和无探诊出血的≥4mm 深牙周袋患者之间进行倾向评分匹配；共生成 62 对匹配对。探诊出血的≥4mm 深牙周袋患者的 HCMV 阳性率（9.7%）高于缺乏探诊出血的≥4mm 深牙周袋患者（0.0%）（P=0.03）。HCMV 存在与探诊出血的≥4mm 深牙周袋显著相关（P=0.03）。HCMV/牙龈卟啉单胞菌 DNA 存在与探诊出血的≥4mm 深牙周袋显著相关（P=0.03）。
口腔 HCMV 和牙龈卟啉单胞菌的合并感染与活动性牙周炎显著相关。此外，口腔 HCMV 与牙龈卟啉单胞菌之间的相互作用可能与牙周病的严重程度有关。
