Co-Infection of Oral and Is Associated with Active Periodontitis in Middle-Aged and Older Japanese People.

can be detected in subgingival sites of patients with periodontitis. However, the association between oral and periodontitis has not been fully elucidated in Japanese adults. The aim of this study is to clarify the relationship between oral infection/co-infection of oral and and periodontitis among middle-aged and older Japanese people. Eighty-six patients (mean age 70.4 years) who visited the Hiroshima University Hospital from April to September 2021 were investigated in this study. Oral swab samples were collected from the tongue surface. and DNA was detected by real-time PCR using specific DNA primer sets. -positive participants were classified into two groups according to the presence or absence of intron insertion of DNA by PCR analysis. was detected in 22 (25.6%) of the 86 patients. Patients in their 80s recorded a higher -positive rate (35.3%) compared with other participants. However, there was no significant association between the positivity rate and clinical parameters such as sex, age, systemic disease, denture use, or oral health status. Of the 22 -positive participants, 10 participants (45.5%) had with intron insertion; 70% of participants who had with intron insertion exhibited ≥6 mm probing depth. / co-infection was found in 12 patients (14%). Importantly, binomial logistic regression analysis revealed that / co-infection was significantly associated with ≥6 mm periodontal pockets with bleeding on probing ( = 0.02). Co-infection of and is involved in active periodontitis in middle-aged and older people.