Department of Internal Medicine, Faculty of Medicine, Suleyman Demirel University, Cunur, Turkey.
Division of Endocrinology, Department of Internal Medicine, Faculty of Medicine, Suleyman Demirel University, Cunur, Turkey -
Minerva Endocrinol (Torino). 2022 Sep;47(3):314-324. doi: 10.23736/S2724-6507.20.03204-6. Epub 2020 Dec 17.
The aim of this study was to evaluate changes in the serum levels of miR-98, miR-184, miR-185, miR-203 and miR-196a-3p in type 2 diabetes mellitus (T2DM) patients with diabetic nephropathy (DN) and to associate the changes in microRNA levels with urinary albumin excretion (UAE).
The study included 35 healthy individuals aged between 18-65 years, 40 T2DM patients with normoalbuminuria, 40 T2DM patients with microalbuminuria, and 35 T2DM patients with macroalbuminuria. Metabolic laboratory parameters, microalbumin levels in 24-hour urine samples were measured in all groups. Serum levels of vascular endothelial growth factor-A (VEGF-A) and transforming growth factor-β (TGF-β) were measured quantitatively by Enzyme-Linked Immunosorbent Assay (ELISA). Circulating miRNA levels were determined by real-time quantitative PCR.
Serum miR-196a-3p levels were reduced in the normoalbuminuria group compared to the healthy control group. In the macroalbuminuria and microalbuminuria groups, miR-196a-3p levels were higher compared to the normoalbuminuria group. In addition, increase in miR-196a-3p levels in the macroalbuminuria group was more prominent than the microalbuminuria group. Serum miR-203 levels were significantly higher in the macroalbuminuria group compared to healthy controls, microalbuminuria and normoalbuminuria groups; these levels were also higher in the microalbuminuria group compared to normoalbuminuria group. In logistic regression analysis, serum miR-196a-3p and miRNA-203 levels were independently correlated to UAE.
Increased serum levels of miR-203 and miR-196a-3p are independent risk factors of UAE which is a marker of DN.
本研究旨在评估血清 miR-98、miR-184、miR-185、miR-203 和 miR-196a-3p 在 2 型糖尿病伴糖尿病肾病（DN）患者中的变化，并将 miRNA 水平的变化与尿白蛋白排泄（UAE）相关联。
本研究纳入了 35 名年龄在 18-65 岁之间的健康个体、40 名无白蛋白尿的 2 型糖尿病患者、40 名微量白蛋白尿的 2 型糖尿病患者和 35 名大量白蛋白尿的 2 型糖尿病患者。所有组均测量代谢实验室参数和 24 小时尿样中的微量白蛋白水平。通过酶联免疫吸附测定（ELISA）定量测量血清血管内皮生长因子-A（VEGF-A）和转化生长因子-β（TGF-β）水平。通过实时定量 PCR 测定循环 miRNA 水平。
与健康对照组相比，正常白蛋白尿组血清 miR-196a-3p 水平降低。在大量白蛋白尿和微量白蛋白尿组中，miR-196a-3p 水平高于正常白蛋白尿组。此外，与微量白蛋白尿组相比，大量白蛋白尿组 miR-196a-3p 水平的升高更为明显。与健康对照组、微量白蛋白尿组和正常白蛋白尿组相比，大量白蛋白尿组血清 miR-203 水平显著升高；与正常白蛋白尿组相比，微量白蛋白尿组血清 miR-203 水平也升高。在逻辑回归分析中，血清 miR-196a-3p 和 miRNA-203 水平与 UAE 独立相关。
血清 miR-203 和 miR-196a-3p 水平升高是 UAE 的独立危险因素，UAE 是 DN 的标志物。
