Department of General Practice, Hubei NO.3 People's Hospital of Jianghan University, Hubei Province, China.
Department of Endocrinology, Hubei NO.3 People's Hospital of Jianghan University, Hubei Province, China.
Ren Fail. 2024 Dec;46(2):2406390. doi: 10.1080/0886022X.2024.2406390. Epub 2024 Oct 8.
Diabetic nephropathy (DN) is one of the most common and serious complications of diabetes. The purpose of this study was to explore the relationship between serum microRNA-338-3p (miR-338-3p) and miR-105-3p and bone metabolic markers in patients with DN at different stages.
A total of 153 patients diagnosed and treated in the Department of Nephrology from July 2020 to October 2021 were selected as the study objects. According to the staging criteria of diabetic nephropathy and 24-h urinary albumin quantitative level, the patients were divided into control group (35 cases), microalbuminuria group (37 cases), clinical stage albuminuria group (27 cases) and renal failure group (54 cases). Gene expressions were measured by real-time fluorescence quantitative PCR. The correlation was analyzed by Spearman. Serum miR-338-3p and miR-150-5p in the prediction of renal failure in DN was analyzed by ROC curve.
The levels of urinary albumin and serum creatinine were markedly increased with the increase of DN stage ( < 0.05). Compared with the microalbuminuria group, the expression levels of serum miR-383-3p, serum miR-105-3p, 25(OH)-D, BGP and PINP were obviously decreased, but the expression of parathyroid hormone (PTH) and type I collagen (β-CTX) was largely increased in clinical proteinuria group ( < 0.05). Compared with the clinical proteinuria group, the expression levels of serum miR-383-3p, serum miR-105-3p, 25(OH)-D, BGP and PINP were largely decreased, but the expression of PTH and β-CTX was obviously increased in the renal failure group ( < 0.05). Spearman correlation results showed that serum expressions of miR-383-3p and miR-105-3p were negatively correlated with PTH and β-CTX, and positively correlated with 25(OH)-D, BGP and PINP ( < 0.05). ROC curve analysis showed that the AUC of serum miR-338-3p and miR-150-5p was 0.896 with the specificity and sensitivity of 96.66% and 73.47%, which had certain predictive value for the occurrence of renal failure in DN.
The expression levels of serum miR-383-3p and miR-105-3p were significantly correlated with bone metabolism markers. The combined test can provide new ideas and insights for the clinical treatment of osteoporosis in DN.
糖尿病肾病（DN）是糖尿病最常见和最严重的并发症之一。本研究旨在探讨不同分期 DN 患者血清微小 RNA-338-3p（miR-338-3p）和 miR-105-3p 与骨代谢标志物的关系。
选取 2020 年 7 月至 2021 年 10 月在我院肾病科诊治的 153 例患者为研究对象。根据糖尿病肾病分期标准和 24 h 尿白蛋白定量水平，将患者分为对照组（35 例）、微量白蛋白尿组（37 例）、临床蛋白尿组（27 例）和肾衰竭组（54 例）。采用实时荧光定量 PCR 法检测基因表达。采用 Spearman 分析进行相关性分析。采用 ROC 曲线分析血清 miR-338-3p 和 miR-150-5p 在预测 DN 肾衰竭中的价值。
随着 DN 分期的增加，尿白蛋白和血肌酐水平显著升高（ < 0.05）。与微量白蛋白尿组相比，临床蛋白尿组血清 miR-383-3p、血清 miR-105-3p、25（OH）-D、BGP 和 PINP 表达水平明显降低，甲状旁腺激素（PTH）和 I 型胶原（β-CTX）表达水平明显升高（ < 0.05）。与临床蛋白尿组相比，肾衰竭组血清 miR-383-3p、血清 miR-105-3p、25（OH）-D、BGP 和 PINP 表达水平明显降低，PTH 和 β-CTX 表达水平明显升高（ < 0.05）。Spearman 相关性分析结果显示，血清 miR-383-3p 和 miR-105-3p 表达与 PTH 和 β-CTX 呈负相关，与 25（OH）-D、BGP 和 PINP 呈正相关（ < 0.05）。ROC 曲线分析显示，血清 miR-338-3p 和 miR-150-5p 的 AUC 为 0.896，特异性和敏感性分别为 96.66%和 73.47%，对 DN 肾衰竭的发生具有一定的预测价值。
血清 miR-383-3p 和 miR-105-3p 的表达水平与骨代谢标志物显著相关。联合检测可为 DN 骨质疏松症的临床治疗提供新的思路和见解。
