Zhang Han, Wang Lulu, Yao Xiaolin, Wang Zonghan, Dou Leina, Su Lihong, Zhao Man, Sun Jing, Zhang Daohong, Wang Jianlong
College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China.
Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning Road, Xining, Qinghai 810008, China.
J Agric Food Chem. 2020 Dec 30;68(52):15509-15515. doi: 10.1021/acs.jafc.0c05972. Epub 2020 Dec 17.
An innovative lateral flow competition immunoassay (LFCIA) for detecting clenbuterol (CL) was developed by employing the advantages of the coomassie brilliant blue (CBB) staining method. An antibody stained by CBB was used both as a recognition reagent and as a chromogenic probe, enabling the simple but sensitive LFCIA of CL. The CBB-based LFCIA exhibited sensitivity for CL with a detection limit of 2 ng mL. Furthermore, this strategy was preliminarily verified by screening for CL in milk, pork tenderloin, and swine liver with recoveries ranging from 81 to 102%. Compared with conventional LFCIAs, the use of CBB as a signal label not only avoided the complicated material synthesis and surface modification process but also simplified the cross-linking with antibodies, meanwhile reducing the steric hindrance and increasing the possibility of immune recognition reactions, which was propitious for the effective utilization of antibodies. Taking advantages of the simplicity, rapidity, and cost-effectiveness, the CBB-based LFCIA may have potential for on-demand monitoring of general harmful small molecules by changing the kind of the staining antibody.
利用考马斯亮蓝（CBB）染色法的优势，开发了一种用于检测克伦特罗（CL）的创新型侧向流动竞争免疫分析（LFCIA）方法。用CBB染色的抗体既用作识别试剂又用作显色探针，实现了简单而灵敏的CL-LFCIA检测。基于CBB的LFCIA对CL表现出敏感性，检测限为2 ng/mL。此外，通过对牛奶、猪里脊肉和猪肝中的CL进行筛查，初步验证了该策略，回收率在81%至102%之间。与传统的LFCIA相比，使用CBB作为信号标记不仅避免了复杂的材料合成和表面修饰过程，还简化了与抗体的交联，同时减少了空间位阻，增加了免疫识别反应的可能性，有利于抗体的有效利用。基于CBB的LFCIA具有简单、快速和成本效益高的优点，通过改变染色抗体的种类，可能具有对一般有害小分子进行按需监测的潜力。
