Sun Ping, Li Xin-Peng, Xin Jie, Xue Tao, Zhang Bo, Liu Yan-Juan
School of Pharmacy, Linyi University, Linyi 276000, China.
Int J Anal Chem. 2022 Apr 26;2022:7067245. doi: 10.1155/2022/7067245. eCollection 2022.
In this study, a colloidal gold immunochromatographic strip was developed for simultaneous detection of rhein and aloe-emodin in rhubarb. The cutoff value, defined as the lowest concentration for which the test line was invisible on the strip, was 50 ng mL for both rhein and aloe-emodin. By contrast, the cutoff value for emodin was 2,000 ng mL. No competitive inhibition was observed up to 5,000 ng mL of physcion, chrysophanol, sennoside A, sennoside B, or rhaponticin. Semiquantitative analyses of the total contents of rhein and aloe-emodin in raw drug materials via our colloidal gold immunochromatographic strips produced results agreeable with those determined by HPLC. Taken together, our findings suggest that the implementation of our colloidal gold immunochromatographic strips provides a rapid one-step method for estimating the total contents of rhein and aloe-emodin, which may represent a powerful tool for quality control of rhubarb.
在本研究中，开发了一种胶体金免疫层析试纸条，用于同时检测大黄中的大黄酸和芦荟大黄素。临界值定义为试纸上检测线不可见的最低浓度，大黄酸和芦荟大黄素的临界值均为50 ng/mL。相比之下，大黄素的临界值为2000 ng/mL。在高达5000 ng/mL的大黄酚、大黄素、番泻苷A、番泻苷B或土大黄苷存在下，未观察到竞争抑制。通过我们的胶体金免疫层析试纸条对原料药中大黄酸和芦荟大黄素的总含量进行半定量分析，结果与高效液相色谱法测定的结果一致。综上所述，我们的研究结果表明，使用我们的胶体金免疫层析试纸条提供了一种快速的一步法来估计大黄酸和芦荟大黄素的总含量，这可能是大黄质量控制的有力工具。
