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sp. nov.,分离自韩国独岛( Dokdo Islands )本土植物的根际土壤。

sp. nov., isolated from the rhizospheric soil of , a plant native to the Dokdo Islands, Republic of Korea.

机构信息

School of Life Sciences, Research Institute for Dok-do & Ulleung-do Island, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea.

School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004612. Epub 2020 Dec 11.

Abstract

KUDC8001 was isolated from the rhizosperic soil of in the Dokdo Islands, Republic of Korea. Strain KUDC8001 was Gram-stain-negative, non-motile and rod-shaped. KUDC8001 was catalase- and oxidase-positive. This strain is capable of growing at 4-37 °C and pH 7.0-8.0 and exhibited optimal growth at 25 °C and pH 7.0. It could be grown in R2A, nutrient agar and ISP2 agar plates. The cell width ranged from 0.7 to 1.0 µm, and length ranged from 2.5 to 5.5 µm. The genomic G+C content was 40.8 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that the strain KUDC8001 belongs to the genus , which is most closely related to the strain HMF7616 (97.5%). The DNA relatedness of KUDC8001 with the type strains of HMF7616, 17 mud1-7 and HMF7605 was ≤80.3 % based on average nucleotide identity calculations and ≤86.9 % based on average amino acid identity calculations. DNA-DNA hybridization values of the strain KUDC8001 with the most closely related strains were 22.1, 24.0 and 24.4 %. Based on its phenotypic, phylogenetic, genetic and chemotaxonomic features, the strain KUDC8001 should be considered as a novel species in the genus , for which we have proposed the name sp. nov. The type strain is KUDC8001 (=KCTC 82078=CGMCC 1.18475).

摘要

KUDC8001 是从韩国独岛 Dokdo 群岛的根际土壤中分离出来的。菌株 KUDC8001 革兰氏阴性,不运动,呈杆状。KUDC8001 过氧化氢酶和氧化酶阳性。该菌株能够在 4-37℃和 pH7.0-8.0 下生长,最适生长温度为 25℃,pH7.0。它可以在 R2A、营养琼脂和 ISP2 琼脂平板上生长。细胞宽度范围为 0.7-1.0μm,长度范围为 2.5-5.5μm。基因组 G+C 含量为 40.8mol%。基于 16S rRNA 基因序列的系统发育分析表明,菌株 KUDC8001 属于 属,与菌株 HMF7616(97.5%)最为密切相关。根据平均核苷酸同源性计算,KUDC8001 与 HMF7616、17 mud1-7 和 HMF7605 型菌株的 DNA 相关性≤80.3%,根据平均氨基酸同源性计算,DNA 相关性≤86.9%。菌株 KUDC8001 与最密切相关菌株的 DNA-DNA 杂交值分别为 22.1%、24.0%和 24.4%。基于其表型、系统发育、遗传和化学分类特征,菌株 KUDC8001 应被视为属中的一个新种,我们建议将其命名为 sp. nov.。模式菌株为 KUDC8001(=KCTC 82078=CGMCC 1.18475)。

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