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sp. nov.,分离自独岛土壤。

sp. nov., isolated from Dokdo Islands soil.

机构信息

School of Life Sciences, Research Institute for Dok-do & Ulleung-do Island, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea.

School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2020 Jan;70(1):16-22. doi: 10.1099/ijsem.0.003689.

DOI:10.1099/ijsem.0.003689
PMID:31513005
Abstract

Strain KUDC5002 was isolated from soil sampled on the Dokdo Islands, Republic of Korea. This bacterial strain was Gram stain-positive, non-motile, rod-shaped, capable of growing at 25-37°C and pH 5.0-12.0, and showed optimal growth at 30 °C and pH 7.0-8.0. Strain KUDC5002 could be grown in tryptic soy broth containing less than 7.0 % NaCl (w/v). The cell width ranged from 0.5 to 0.6 µm and length ranged from 0.8 to 1.0 µm. Strain KUDC5002 was catalase- and oxidase-positive. Its genomic G+C content was 72.2 mol%. Its major fatty acids were C 9 (17.3 %), iso-C (16.0 %) and iso-C (11.4 %). Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain KUDC5002 belongs to the genus and is most closely related to strain DCY24 (97.0 %). Based on its phenotypic, phylogenetic, genetic and chemotaxonomic features, strain KUDC5002 should be considered a novel species in the genus , for which we have proposed the name sp. nov. The type strain is KUDC5002 (=KCTC 39855=DSM 106604).

摘要

菌株 KUDC5002 是从韩国独岛土壤中分离出来的。该细菌为革兰氏阳性、非运动性、杆状,能在 25-37°C 和 pH5.0-12.0 下生长,最适生长温度为 30°C,最适 pH 为 7.0-8.0。KUDC5002 菌株能在含少于 7.0%(w/v)NaCl 的胰蛋白胨大豆肉汤中生长。细胞宽度为 0.5-0.6μm,长度为 0.8-1.0μm。KUDC5002 菌株过氧化氢酶和氧化酶阳性。其基因组 G+C 含量为 72.2mol%。其主要脂肪酸为 C9(17.3%)、iso-C(16.0%)和 iso-C(11.4%)。基于 16S rRNA 基因序列的系统发育分析表明,菌株 KUDC5002 属于 属,与菌株 DCY24(97.0%)最为密切相关。基于其表型、系统发育、遗传和化学分类特征,菌株 KUDC5002 应被视为 属的一个新种,我们建议将其命名为 sp. nov.。模式菌株为 KUDC5002(=KCTC 39855=DSM 106604)。

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