sp. nov., a novel actinobacterium isolated from rhizospheric soil of the wild plant .

An actinobacterial strain, designated KUDC0627, was isolated from rhizospheric soil that contained on the Dokdo Islands, Republic of Korea. Cells were Gram-stain-positive, facultative anaerobic, non-motile and non-endospore-forming cocci. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KUDC0627 belongs to the genus and is most closely related to DSM 21800 (98.5 %), DSM 100019 (97.7 %) and Gsoil 633 (96.5 %). The average nucleotide identity scores and average amino acid identity values were all below the 95.0 % cut-off point. DNA-DNA hybridization, using the Genome-to-Genome Distance Calculator, estimated that there is 22.3 % DNA relatedness between KUDC0627 and DSM 21800. The genomic DNA G+C content was 66.9 mol%. The major menaquinone was MK-9(H) and the major diagnostic diamino acid in the cell-wall peptidoglycan was ll-diaminopimelic acid. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unidentified lipids. The major cellular fatty acids were iso-C, anteiso-C and iso-C. Based on phenotypic, chemotaxonomic, and phylogenetic data, strain KUDC0627 (=KCTC 39853=JCM 32702) represents a novel species, for which the name sp. nov. is proposed.