Gichner T, Velemínský J
Institute of Experimental Botany, Praha, Czechoslovakia.
Mutagenesis. 1986 Mar;1(2):107-9. doi: 10.1093/mutage/1.2.107.
The organic solvents dimethylsulphoxide (DMSO), acetone, ethanol and dimethylformamide inhibited the mutagenic activity of the promutagens dimethylnitrosamine and methylbutylnitrosamine in a higher plant Arabidopsis thaliana. In contrast, the direct-acting mutagens N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and N-methyl-N-nitrosourea (MNU) were not affected by the organic solvents (with the exception of DMSO). The effect of DMSO on MNNG- and MNU-mutagenesis is explained by its effect on the pH of the mutagenic solution. The results support the assumption that the activation of promutagenic nitrosamines in A. thaliana proceeds by the same, or similar, mechanisms to that in 'animal' activation.
有机溶剂二甲基亚砜（DMSO）、丙酮、乙醇和二甲基甲酰胺抑制了高等植物拟南芥中前诱变剂二甲基亚硝胺和甲基丁基亚硝胺的诱变活性。相比之下，直接作用诱变剂N-甲基-N'-硝基-N-亚硝基胍（MNNG）和N-甲基-N-亚硝基脲（MNU）不受有机溶剂影响（DMSO除外）。DMSO对MNNG和MNU诱变作用的影响是由其对诱变溶液pH值的影响来解释的。这些结果支持了这样一种假设，即拟南芥中前诱变亚硝胺的活化过程与“动物”活化过程相同或相似。
