Mori Y, Yamazaki H, Toyoshi K, Emi Y, Uchida K, Tsutsumi M, Konishi Y
Mutat Res. 1985 Apr;142(4):153-8. doi: 10.1016/0165-7992(85)90015-6.
The influence of organic solvents on the mutagenicity of 11 N-nitrosamines was examined in Salmonella typhimurium TA100 using the Ames's liquid incubation assay in the presence of rat-liver S9. The mutagenic activities of N-nitrosodimethylamine, N-nitrosodiethylamine, 6 oxidative derivatives of N-nitrosopropylamine and N-nitroso-2,6-dimethylmorpholine were considerably decreased by addition of dimethyl sulfoxide, dimethyl formamide, acetone, 95% ethanol or acetonitrile, which are recommended for use as solvents in the assay by Ames's group, to the incubation mixture. The mutagenic activities of N-nitrosodipropylamine and N-nitrosodibutylamine, which are barely soluble in water, were also suppressed by increasing concentrations of dimethyl sulfoxide. These organic solvents did not appear to exert their influence by desmutagenic and antimutagenic actions. In contrast, the recoveries of unmetabolized carcinogens from preincubation mixtures and from agar plates were significantly higher in the presence of organic solvents than in their absence. The results indicate that the inhibitory effect is a result of interference with the process of metabolic activation by liver S9.
在鼠伤寒沙门氏菌TA100中，利用艾姆斯氏液体培养试验，在大鼠肝脏S9存在的情况下，研究了有机溶剂对11种N - 亚硝胺致突变性的影响。将二甲基亚砜、二甲基甲酰胺、丙酮、95%乙醇或乙腈添加到培养混合物中后，N - 亚硝基二甲胺、N - 亚硝基二乙胺、N - 亚硝基丙胺的6种氧化衍生物以及N - 亚硝基 - 2,6 - 二甲基吗啉的致突变活性显著降低，艾姆斯团队推荐将这些有机溶剂用于该试验的溶剂。N - 亚硝基二丙胺和N - 亚硝基二丁胺几乎不溶于水，随着二甲基亚砜浓度的增加，它们的致突变活性也受到抑制。这些有机溶剂似乎并非通过去诱变和抗诱变作用发挥影响。相反，在有机溶剂存在的情况下，预培养混合物和琼脂平板中未代谢致癌物的回收率明显高于不存在有机溶剂的情况。结果表明，抑制作用是肝脏S9干扰代谢活化过程的结果。
