Post-Graduation Program in Biological Sciences: Toxicological Biochemistry, Center of Natural and Exact Sciences of the Federal University of Santa Maria, Santa Maria, RS, Brazil.
Graduate Program in Biomedical Sciences, Federal University of Fronteira Sul, Chapecó, SC, Brazil.
Clin Biochem. 2021 Mar;89:63-69. doi: 10.1016/j.clinbiochem.2020.12.007. Epub 2020 Dec 14.
Metabolic syndrome (MetS) is a disorder that is closely associated with risk factors that increase the chance of atherosclerosis and cardiovascular diseases. We demonstrate the presence of inflammation and oxidative stress in patients with MetS through levels of antioxidants and oxidative and inflammatory markers, in order to determine influential variables in therapy.
In this study, lipid peroxidation, carbonylated protein content and enzymatic and non-enzymatic antioxidants were evaluated in samples obtained from 30 patients with MetS and 30 control patients. In addition, acetylcholinesterase (AChE) activity, C-reactive protein (CRP) and uric acid (UA) levels were determined to investigate the inflammatory process in patients with MetS.
Our results demonstrated an increase in the levels of oxidative markers, such as substances reactive to thiobarbituric acid (TBARS) and carbonyl protein. In addition, a decrease in the defense of non-enzymatic antioxidants, such as levels of vitamin C and glutathione (GSH) in patients with MetS. As for inflammatory markers, CRP and UA were increased in patients with MetS. Finally, activation of the cholinergic anti-inflammatory pathway was observed due to decreased AchE activity in patients with MetS.
The analyzes indicated oxidative stress, together with a reduction in the levels of antioxidant enzymes, corroborating the high consumption of these proteins. In addition, inflammation and activation of the cholinergic anti-inflammatory pathway was observed by the AChE analysis. Thus, the activation of this pathway can be studied as a possible route to a potential therapy. In addition, the markers AChE, CRP and UA may be used as a focus for the treatment of MetS.
代谢综合征（MetS）是一种与增加动脉粥样硬化和心血管疾病风险的因素密切相关的疾病。我们通过抗氧化剂和氧化应激及炎症标志物的水平，在 MetS 患者中证明了炎症和氧化应激的存在，以确定治疗中的影响变量。
在这项研究中，我们评估了 30 名 MetS 患者和 30 名对照患者样本中的脂质过氧化、羰基蛋白含量以及酶和非酶抗氧化剂。此外，还测定了乙酰胆碱酯酶（AChE）活性、C 反应蛋白（CRP）和尿酸（UA）水平，以研究 MetS 患者的炎症过程。
我们的结果表明，氧化标志物水平升高，如丙二醛（TBARS）和羰基蛋白反应物质。此外，MetS 患者的非酶抗氧化剂防御能力下降，如维生素 C 和谷胱甘肽（GSH）水平下降。至于炎症标志物，CRP 和 UA 在 MetS 患者中增加。最后，由于 MetS 患者 AchE 活性降低，观察到胆碱能抗炎途径的激活。
分析表明氧化应激与抗氧化酶水平降低有关，这证实了这些蛋白质的高消耗。此外，AChE 分析表明存在炎症和胆碱能抗炎途径的激活。因此，该途径的激活可以作为潜在治疗的一个可能途径进行研究。此外，AChE、CRP 和 UA 标志物可用作 MetS 治疗的重点。
