Cornell University, Ithaca, NY, United States.
U.S. Geological Survey, Western Fisheries Research Center (WFRC), Seattle, WA, United States.
J Invertebr Pathol. 2021 Jan;178:107519. doi: 10.1016/j.jip.2020.107519. Epub 2020 Dec 16.
Nuclear inclusion X (NIX) is a gamma proteobacteria that infects the nuclei of gill epithelial cells in Pacific razor clams. NIX has been associated with clam die-offs in coastal Washington. A quantitative PCR (qPCR) assay was developed to detect NIX in Pacific razor clams, and assay specificity was confirmed by chromogenic in situ hybridization (CISH). Both tests were applied to evaluate NIX infections in wild Pacific razor clams collected during spring 2019. Consistent with results from earlier histopathological assessments, qPCR and CISH indicated 100% prevalence in razor clams from two Washington beaches and 0% prevalence from two Alaskan beaches.
核包含物 X（NIX）是一种γ变形菌，感染太平洋贻贝鳃上皮细胞的细胞核。NIX 与华盛顿沿海地区的贻贝死亡有关。已经开发了一种定量 PCR（qPCR）检测太平洋贻贝中的 NIX 的方法，并通过显色原位杂交（CISH）验证了检测方法的特异性。这两种方法都用于评估 2019 年春季采集的野生太平洋贻贝中的 NIX 感染情况。与之前的组织病理学评估结果一致，qPCR 和 CISH 表明，来自华盛顿两个海滩的贻贝的患病率为 100%，来自阿拉斯加两个海滩的贻贝的患病率为 0%。
