Chr. Hansen A/S, Animal Health Innovation, Bøge Allé 10-12, 2970 Hørsholm, Denmark; Aarhus University, Department of Animal Science, Blichers Allé 20, 8830 Tjele, Denmark.
Chr. Hansen A/S, Animal Health Innovation, Bøge Allé 10-12, 2970 Hørsholm, Denmark.
J Microbiol Methods. 2021 Jan;180:106126. doi: 10.1016/j.mimet.2020.106126. Epub 2020 Dec 14.
Enterotoxigenic Escherichia coli (ETEC), being the major cause of post-weaning diarrhoea (PWD) in newly weaned piglets, induces poor performance and economic losses in pig production. This functional in vitro screening study investigated probiotic strains for use in suckling piglets as a prophylactic strategy towards PWD. Nine strains were evaluated based on their ability to: enhance intestinal epithelial barrier function, reduce adherence of ETEC F18 to intestinal cells, inhibit growth of ETEC F18, and grow on porcine milk oligosaccharides. Strains included in the screening were of the species Lactobacillus, Enterococcus, Bifidobacterium and Bacillus. Our in vitro screening demonstrated genus-, species and strain-specific differences in the mode of action of the tested probiotic strains. Some of the tested bifidobacteria were able to grow on the two porcine milk oligosaccharides, 3'-sialyllactose sodium salt (3'SL) and Lacto-N-neotetraose (LNnT), whereas most lactic acid bacteria strains and both Bacillus subtilis strains failed to do so. All probiotic strains inhibited growth of ETEC F18 on agar plates. All but the bifidobacteria reduced binding of ETEC F18 to Caco-2 cell monolayers, with the Enterococcus faecium strain having the most profound effect. All three lactic acid bacteria and Bifidobacterium animalis subsp. lactis counteracted the ETEC F18-induced permeability across Caco-2 cell monolayers with the E. faecium strain exhibiting the most pronounced protective effect. The findings from this in vitro screening study indicate that, when selecting probiotic strains for suckling piglets as a prophylactic strategy towards PWD, it would be advantageous to choose a multi-species product including strains with different modes of action in order to increase the likelihood of achieving beneficial effects in vivo.
肠产毒性大肠杆菌（ETEC）是新生仔猪断奶后腹泻（PWD）的主要原因，它会导致猪生产性能下降和经济损失。这项体外功能筛选研究调查了益生菌菌株，以作为预防 PWD 的新生仔猪策略。基于增强肠道上皮屏障功能、减少 ETEC F18 对肠道细胞的粘附、抑制 ETEC F18 生长和在猪乳低聚糖上生长的能力，评估了 9 种菌株。筛选的菌株包括乳杆菌、肠球菌、双歧杆菌和芽孢杆菌。我们的体外筛选表明，测试益生菌菌株的作用模式存在属、种和菌株特异性差异。一些双歧杆菌能够在两种猪乳低聚糖，3'-唾液酸乳糖钠盐（3'SL）和乳-N-新四糖（LNnT）上生长，而大多数乳酸菌菌株和两种枯草芽孢杆菌菌株都不能。所有益生菌菌株都能抑制琼脂平板上 ETEC F18 的生长。除双歧杆菌外，所有益生菌菌株都能减少 ETEC F18 与 Caco-2 细胞单层的结合，其中肠球菌 faecium 菌株的效果最显著。三种乳酸菌和双歧杆菌乳亚种。乳糖都能拮抗 ETEC F18 诱导的 Caco-2 细胞单层通透性，其中肠球菌 faecium 菌株表现出最明显的保护作用。这项体外筛选研究的结果表明，在选择益生菌菌株作为预防 PWD 的新生仔猪策略时，选择具有不同作用模式的多物种产品将是有利的，以增加在体内获得有益效果的可能性。
