葛根素通过抑制大鼠模型中的炎症缓解冠心病。
Puerarin alleviates coronary heart disease via suppressing inflammation in a rat model.
机构信息
Department of Cardiology, The Second Affiliated Hospital of Soochow University, Gusu District, Suzhou, Jiangsu Province 215004, China.
Department of Cardiology, The Second Affiliated Hospital of Soochow University, Gusu District, Suzhou, Jiangsu Province 215004, China.
出版信息
Gene. 2021 Mar 1;771:145354. doi: 10.1016/j.gene.2020.145354. Epub 2020 Dec 15.
BACKGROUND
Puerarin shows inhibitory effects on inflammation in chronic heart failure (CHF), but its efficacy in coronary heart disease (CHD) remained vague.
METHODS
Rat CHD model was constructed, and serum parameters were determined using a blood liquid biochemical analyzer. Also, contents of creatine kinase (CK), creatine kinase MB isoenzyme (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin (cTnT) were measured using colorimetry. Histological examination was conducted with Hematoxylin-Eosin (H&E) staining, and cardiac function was assessed by Echocardiography. Cell apoptosis was detected using Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Relative expressions were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed.
RESULTS
In CHD rats, the levels of TC, LDL and TG and the expressions of matrix metalloproteinase-9 (MMP-9), CD40 ligand (CD40L), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were increased while HDL level was decreased, accompanied with inflammatory cell infiltration and cardiac malfunction. Also, the contents of CK, CK-MB, LDH and cTnT, the percentage of apoptotic cells, the expressions of Bcl-2 associated X protein (Bax), cleaved Caspase-3, TNF-α, Interleukin-β (IL-β), IL-6 and Lipoprotein-associated Phospholipase A2 (Lp-PLA2) expressions and the levels of oxidized-(ox-)LDL and malondialdehyde (MDA) were upregulated, while the level of super oxidase dismutase (SOD) and the expressions of B cell lymphoma-2 (Bcl-2) and vascular endothelial growth factor (VEGF) were downregulated. However, Puerarin ameliorated the effects of CHD model construction, suppressed nuclear factor-(NF-)κB expression, and enhanced the expressions of Farnesoid X Receptor (FXR), phosphorylated-AKT (p-AKT) and phosphorylated-signal transducer and activator of transcription 3 (p-STAT3).
CONCLUSION
Puerarin alleviated CHD in rats via inhibiting inflammation, providing possible method for CHD treatment.
背景
葛根素对慢性心力衰竭（CHF）中的炎症具有抑制作用，但在冠心病（CHD）中的疗效尚不清楚。
方法
构建大鼠 CHD 模型，使用血液液体生化分析仪测定血清参数。此外，使用比色法测定肌酸激酶（CK）、肌酸激酶同工酶 MB（CK-MB）、乳酸脱氢酶（LDH）和心肌肌钙蛋白 T（cTnT）的含量。苏木精-伊红（H&E）染色进行组织学检查，超声心动图评估心功能。使用末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记（TUNEL）法检测细胞凋亡。根据需要使用定量实时聚合酶链反应（qRT-PCR）和 Western blot 测定相对表达量。
结果
在 CHD 大鼠中，TC、LDL 和 TG 水平升高，基质金属蛋白酶-9（MMP-9）、CD40 配体（CD40L）、肿瘤坏死因子-α（TNF-α）和 C 反应蛋白（CRP）的表达增加，而 HDL 水平降低，伴有炎症细胞浸润和心功能障碍。此外，CK、CK-MB、LDH 和 cTnT 的含量、凋亡细胞的百分比、Bcl-2 相关 X 蛋白（Bax）、裂解 Caspase-3、TNF-α、白细胞介素-β（IL-β）、白细胞介素-6（IL-6）和脂蛋白相关磷脂酶 A2（Lp-PLA2）的表达以及氧化型-LDL（ox-LDL）和丙二醛（MDA）的水平上调，而超氧化物歧化酶（SOD）的水平和 B 细胞淋巴瘤-2（Bcl-2）和血管内皮生长因子（VEGF）的表达下调。然而，葛根素改善了 CHD 模型构建的效果，抑制了核因子-κB 的表达，并增强了法尼醇 X 受体（FXR）、磷酸化-AKT（p-AKT）和磷酸化信号转导和转录激活因子 3（p-STAT3）的表达。
结论
葛根素通过抑制炎症减轻大鼠 CHD，为 CHD 治疗提供了可能的方法。
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