Effect of Nitrogen on Phosphine-Susceptible and -Resistant Populations of Stored Product Insects.

In this study, we evaluated nitrogen treatment on phosphine-resistant field and -susceptible laboratory populations of different stored product beetles. Nine trials were conducted in commercial nitrogen chambers with the O level set at 1.0%. Two different temperatures-i.e., 28 and 40 °C-and three exposure intervals-i.e., 2.5, 3 and 9 d-were used in our tests. Adults of the sawtoothed grain beetle, (L.) (Coleoptera: Silvanidae); the red flour beetle, (Herbst) (Coleoptera: Tenebrionidae); and the rice weevil, (L.) (Coleoptera: Curculionidae) were used in the trials. The insects were placed in vials with different commodities per species and population, and their mortality was measured after the termination of each trial. Then, the vials were kept in incubator chambers at 25 °C and 65% relative humidity for 65 d to measure progeny production. Complete parental mortality was observed in all cases for and , but there was some survival for at 28 °C and 3 d of exposure. In general, progeny production was completely (100%) suppressed, with some exceptions for all species and populations. The results indicate that low oxygen is effective for all species tested, regardless of their resistance status to phosphine, and can be further adopted as an alternative method to mitigate resistance in stored product beetles.