Department of Traditional Chinese Medicine, Wuhan Fifth Hospital, Wuhan.
School of Physical Education, Wuhan Business University, Wuhan.
Eur J Histochem. 2020 Oct 1;64(s2):3170. doi: 10.4081/ejh.2020.3170.
Considerable evidence has verified that microRNAs (miRNAs) play important roles in various cellular processes including differentiation. However, the regulatory roles of miRNAs involved in the differentiation of induced pluripotent stem cells (iPSC) into lung epithelial cells are still unknown. In this study, we first evaluated the current protocols to differentiate iPSC into alveolar epithelial type II (AEC II) cells, but the efficiency is low. We next identified that miR-22 can efficiently enhance the differentiation of iPSC into AEC II cells under the stimulation of proper growth factors and growing on appropriate matrix. Moreover, the AEC II cells generated from iPSC with miR-22 overexpression can proliferate and secrete lung surfactant. Here, we discovered a previously unknown interaction between miR-22 and iPSC differentiation but also provide a potential target for the effective derivation of AEC II from iPSCs for cell-based therapy.
大量证据已经证实，微小 RNA（miRNA）在包括分化在内的各种细胞过程中发挥着重要作用。然而，miRNA 在诱导多能干细胞（iPSC）分化为肺上皮细胞过程中的调节作用尚不清楚。在这项研究中，我们首先评估了目前将 iPSC 分化为肺泡上皮细胞 II 型（AEC II）细胞的方案，但效率较低。接下来，我们发现 miR-22 可以在适当的生长因子刺激和适当基质上生长的情况下，有效地促进 iPSC 分化为 AEC II 细胞。此外，过表达 miR-22 的 iPSC 产生的 AEC II 细胞可以增殖并分泌肺表面活性剂。在这里，我们发现了 miR-22 与 iPSC 分化之间以前未知的相互作用，但也为有效从 iPSC 中分离 AEC II 细胞用于细胞治疗提供了一个潜在的靶点。
