Mello M L, Pudney M
Dept. of Cell Biology, UNICAMP, Campinas (SP), Brazil.
Genetica. 1987 Oct 15;74(2):131-6. doi: 10.1007/BF00055224.
The cytophotometric evaluation of the Feulgen-DNA content of the BTC-32 cells at passage 160 after 6 days of growth demonstrated that polyploidy is relatively frequent in this cell line. 4C values were assumed to pertain to diploid nuclei at the S or G2 phases but also to polyploid nuclei at the G1 phase. Polyploidy in 4C nuclei is assumed to be attained by endomitosis. However, there are morphological indications that polyploidization in cells with an 8C-128C Feulgen-DNA content could result from cellular and nuclear fusions, possibly mediated by viral particles present in the cell culture. Micronucleation was also frequent; it was assumed to be promoted by viral action or deficiency in some culture medium nutrient. These nuclear characteristics should be considered when using the BTC-32 cell line for monitoring the action of infective agents or their products.
对生长6天后第160代的BTC - 32细胞进行福尔根DNA含量的细胞光度测定评估表明，多倍体在该细胞系中相对常见。4C值被认为既适用于处于S期或G2期的二倍体细胞核，也适用于处于G1期的多倍体细胞核。4C细胞核中的多倍体被认为是通过核内有丝分裂实现的。然而，有形态学迹象表明，福尔根DNA含量为8C - 128C的细胞中的多倍体化可能是由细胞和细胞核融合导致的，可能由细胞培养物中存在的病毒颗粒介导。微核形成也很常见；据推测，这是由病毒作用或某些培养基营养物质缺乏所促进的。在使用BTC - 32细胞系监测感染因子或其产物的作用时，应考虑这些核特征。
