Pudney M, Lanar D
Ann Trop Med Parasitol. 1977 Mar;71(1):109-18. doi: 10.1080/00034983.1977.11687167.
The establishment of a cell line from embryonic tissues of the triatomine bug, Triatoma infestans is described. The cell line consists of three cell types which are described, and has a population doubling time of 48 hours during the logarithmic phase of growth. A proportion of the cells remain in the medium as floaters. Of the chromosome preparations that could be counted, the majority contained the diploid (2n = 22) number. The cell line has undergone 129 subcultures and has been maintained for over three years. The potential use of these cells in the study of Trypanosoma cruzi is described.
本文描述了从锥蝽（Triatoma infestans）胚胎组织建立细胞系的过程。该细胞系由三种细胞类型组成，并对其进行了描述，在对数生长期其群体倍增时间为48小时。一部分细胞以漂浮细胞的形式留在培养基中。在可计数的染色体标本中，大多数含有二倍体（2n = 22）数目。该细胞系已传代129次，并已维持了三年多。文中还描述了这些细胞在克氏锥虫研究中的潜在用途。
