Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Straße 9, 97078, Würzburg, Germany.
Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany.
Sci Rep. 2020 Dec 17;10(1):22196. doi: 10.1038/s41598-020-78832-y.
Neurodegenerative diseases show an increase in prevalence and incidence, with the most prominent example being Alzheimer's disease. DNA damage has been suggested to play a role in the pathogenesis, but the exact mechanisms remain elusive. We enrolled 425 participants with and without neurodegenerative diseases and analyzed DNA damage in the form of micronuclei in buccal mucosa samples. In addition, other parameters such as binucleated cells, karyolytic cells, and karyorrhectic cells were quantified. No relevant differences in DNA damage and cytotoxicity markers were observed in patients compared to healthy participants. Furthermore, other parameters such as lifestyle factors and diseases were also investigated. Overall, this study could not identify a direct link between changes in buccal cells and neurogenerative diseases, but highlights the influence of lifestyle factors and diseases on the human buccal cytome.
神经退行性疾病的患病率和发病率呈上升趋势，其中最突出的例子是阿尔茨海默病。有人认为 DNA 损伤在发病机制中起作用，但确切的机制仍不清楚。我们招募了 425 名患有和不患有神经退行性疾病的参与者，并分析了口腔黏膜样本中微核形式的 DNA 损伤。此外，还定量了双核细胞、核溶解细胞和核碎裂细胞等其他参数。与健康参与者相比，患者的 DNA 损伤和细胞毒性标志物没有明显差异。此外，还研究了其他参数，如生活方式因素和疾病。总的来说，这项研究未能确定口腔细胞变化与神经退行性疾病之间的直接联系，但强调了生活方式因素和疾病对人类口腔细胞的影响。
