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丁香酚通过Nrf2保护细胞免受氧化应激。

Eugenol protects cells against oxidative stress via Nrf2.

作者信息

Ma Leina, Liu Jia, Lin Qian, Gu Yuchao, Yu Wengong

机构信息

Key Laboratory of Marine Drugs (Ministry of Education), Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong 266003, P.R. China.

Qingdao Cancer Institute, College of Medicine, Qingdao University, Qingdao, Shandong 266021, P.R. China.

出版信息

Exp Ther Med. 2021 Feb;21(2):107. doi: 10.3892/etm.2020.9539. Epub 2020 Nov 27.

DOI:10.3892/etm.2020.9539
PMID:33335570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7739850/
Abstract

Eugenol is a naturally occurring compound that is present in a variety of plants and has previous been demonstrated to exert a number of bioactivities. However, the potential effects of Eugenol on cellular protection against oxidative stress remain poorly understood. In the present study, HEK-293 cells and the mouse fibroblast cell line NIH-3T3 cells were used as models to explore the effects of eugenol on HO-induced damage. Among the three natural compounds tested, namely eugenol, methyleugenol and acetyleugenol, eugenol was found to increase the transcriptional activity and expression level of nuclear factor erythroid 2-related factor 2 (Nrf2), a central regulator of cellular responses to oxidative stress, in a dose-dependent manner. The mRNA levels of Nrf2 target genes glutamate-cysteine ligase modifier regulatory subunit and glutathione S-transferase A1, were also found to be upregulated following eugenol treatment. Further study revealed that eugenol enhanced the stabilization and nuclear translocation of Nrf2. Additionally, treatment with eugenol was found to reduce intracellular ROS levels while increasing cellular resistance to HO, in a manner that was dependent on Nrf2. In conclusion, data from the present study suggest that eugenol is a protective agent against oxidative stress that exerts its effects through a Nrf2-dependent pathway, rendering eugenol and its derivatives to be promising candidates for the future development of antioxidants.

摘要

丁香酚是一种天然存在的化合物，存在于多种植物中，此前已被证明具有多种生物活性。然而，丁香酚对细胞抗氧化应激保护作用的潜在影响仍知之甚少。在本研究中，以人胚肾293细胞（HEK-293细胞）和小鼠成纤维细胞系NIH-3T3细胞为模型，探讨丁香酚对过氧化氢（HO）诱导损伤的影响。在所测试的三种天然化合物，即丁香酚、甲基丁香酚和乙酰丁香酚中，发现丁香酚能以剂量依赖的方式增加核因子红细胞2相关因子2（Nrf2）的转录活性和表达水平，Nrf2是细胞对氧化应激反应的核心调节因子。丁香酚处理后，还发现Nrf2靶基因谷氨酸-半胱氨酸连接酶修饰调节亚基和谷胱甘肽S-转移酶A1的mRNA水平上调。进一步研究表明，丁香酚增强了Nrf2的稳定性和核转位。此外，发现丁香酚处理可降低细胞内活性氧水平，同时增加细胞对HO的抗性，且这种作用依赖于Nrf2。总之，本研究数据表明，丁香酚是一种抗氧化应激保护剂，通过Nrf2依赖途径发挥作用，使丁香酚及其衍生物有望成为未来抗氧化剂开发的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98fd/7739850/1aa9623775e8/etm-21-02-09539-g10.jpg
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