Łęgosz Paweł, Sarzyńska Sylwia, Pulik Łukasz, Kotrych Daniel, Małdyk Paweł
Department of Orthopaedics and Traumatology, 1st Faculty of Medicine, Medical University of Warsaw, Warsaw, Poland.
Department of Orthopaedics, Traumatology and Orthopaedic Oncology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
Open Med (Wars). 2020 Apr 21;15(1):366-375. doi: 10.1515/med-2020-0402. eCollection 2020.
Osteoarthritis (OA) is a common medical problem leading to chronic pain and physical disability among the world's population. Analyzing the molecular background of the degenerative arthritis creates the potential for developing novel targeted methods of treatment. Fifty samples of meniscus, anterior cruciate ligaments (ACLs) and articular surfaces were collected from patients who underwent total knee arthroplasty in 2016. Enzyme-linked immunosorbent assay was used to assess the levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF), transforming growth factor-β1 and LUMINEX for MMP-1, MMP-2, MMP-3, MMP-9 and MMP-13. The collected data were correlated with the severity of radiological OA, demographic data and clinical scales. Strong positive correlations in the concentration of metalloproteinases and proinflammatory cytokines, TNF-α (MMP-2 and MMP-13) and IL-6 (MMP-13), were identified. MMP-13 had a positive correlation with the concentration of MMP-1, MMP-2 and MMP-9. Negative correlation coefficient exists between clinical conditions measured with the Western Ontario and McMaster Universities Osteoarthritis Index scale and the level of TNF-α and MMP-1. The TNF-α concentration was lower in the cartilage of the articular surface among patients who took non-steroidal anti-inflammatory drugs periodically. The decrease in MMP-2 in the cartilage of the articular surface corresponded with the severity of radiological OA on the Kellgren-Lawrence scale. Current treatment methods for OA do not stop disease progression. Identifying signaling pathways and molecular particles engaged in OA and their correlations with the patient's clinical condition brings new therapeutic possibilities.
骨关节炎（OA）是一个常见的医学问题，在全球人口中导致慢性疼痛和身体残疾。分析退行性关节炎的分子背景为开发新的靶向治疗方法创造了潜力。2016年，从接受全膝关节置换术的患者中收集了50份半月板、前交叉韧带（ACL）和关节表面样本。采用酶联免疫吸附测定法评估白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）、转化生长因子-β1的水平，并使用LUMINEX法检测基质金属蛋白酶-1、基质金属蛋白酶-2、基质金属蛋白酶-3、基质金属蛋白酶-9和基质金属蛋白酶-13。收集的数据与放射学OA的严重程度、人口统计学数据和临床量表相关。确定了金属蛋白酶与促炎细胞因子、TNF-α（基质金属蛋白酶-2和基质金属蛋白酶-13）和IL-6（基质金属蛋白酶-13）浓度之间的强正相关。基质金属蛋白酶-13与基质金属蛋白酶-1、基质金属蛋白酶-2和基质金属蛋白酶-9的浓度呈正相关。用西安大略和麦克马斯特大学骨关节炎指数量表测量的临床状况与TNF-α和基质金属蛋白酶-1水平之间存在负相关系数。定期服用非甾体抗炎药的患者关节表面软骨中的TNF-α浓度较低。关节表面软骨中基质金属蛋白酶-2的降低与Kellgren-Lawrence量表上放射学OA的严重程度相对应。目前OA的治疗方法无法阻止疾病进展。识别参与OA的信号通路和分子颗粒及其与患者临床状况的相关性带来了新的治疗可能性。
