Chongqing Research Center for Pharmaceutical Engineering, Chongqing Medical University, Chongqing, China.
Biochemistry and Molecular Biology Laboratory, Experimental Teaching and Management Center, Chongqing Medical University, Chongqing, China.
Methods Mol Biol. 2021;2211:57-68. doi: 10.1007/978-1-0716-0943-9_5.
Catanionic nanovesicles are attractive as a novel class of delivery vehicle because they can increase the stability, adsorption, and cellular uptake of a broad range of drugs. These hybrid lipid nanocarriers consist of solid and liquid lipids, which are biocompatible and biodegradable. Since liquid lipid is added to the nanocarrier, the lipids are present in a crystalline defect or amorphous structure state. As a result, hybrid lipid nanocarriers have a higher drug loading capability and suffer less drug leakage during preparation and storage compared to the pure lipid nanocarriers. Catanionic nanovesicles have been shown to increase stability, adsorption, cellular uptake, apoptosis induction, tumor cell cytotoxicity, and antitumorigenic effect, making it a highly desirable vehicle for drug delivery. For example, the anticancer compound curcumin (CC) have shown great promise to cure cancers such as lung cancer, breast cancer, stomach cancer, and colon cancer. However, like many potential antitumor drugs, CC on its own has poor water solubility, easy photodegradation, chemical instability, low bioavailability, rapid metabolism, and fast systematic clearance, which severely limits its clinical applications. In this chapter, we demonstrate the use of catanionic nanovesicles to improve the bioavailability and efficacy of CC for anticancer applications. This technique can be easily adapted for delivery and evaluation of other bioactive compounds.
混合双离子囊泡作为一种新型的给药载体具有吸引力，因为它们可以提高广泛的药物的稳定性、吸附性和细胞摄取率。这些混合脂质纳米载体由固体和液体脂质组成，具有生物相容性和可生物降解性。由于向纳米载体中添加了液体脂质，因此脂质处于晶体缺陷或无定形结构状态。因此，与纯脂质纳米载体相比，混合脂质纳米载体具有更高的载药能力，并且在制备和储存过程中药物泄漏较少。已经证明混合双离子囊泡可以提高稳定性、吸附性、细胞摄取率、细胞凋亡诱导、肿瘤细胞细胞毒性和抗肿瘤作用，使其成为一种非常理想的药物递送载体。例如，抗癌化合物姜黄素 (CC) 已显示出治疗肺癌、乳腺癌、胃癌和结肠癌等癌症的巨大潜力。然而，像许多潜在的抗肿瘤药物一样，CC 本身水溶性差、容易光降解、化学不稳定、生物利用度低、代谢快、系统清除快，严重限制了其临床应用。在本章中，我们展示了使用混合双离子囊泡来提高 CC 的生物利用度和抗癌效果。这种技术可以很容易地适应其他生物活性化合物的传递和评估。
