Department of Pharmacy, National University of Singapore, Singapore, Singapore.
NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), Singapore, Singapore.
Methods Mol Biol. 2021;2211:147-170. doi: 10.1007/978-1-0716-0943-9_11.
Cell-derived Drug Delivery Systems (DDSs), particularly exosomes, have grown in popularity and have been increasingly explored as novel DDSs, due to their intrinsic targeting capabilities. However, clinical translation of exosomes is impeded by the tedious isolation procedures and poor yield. Cell-derived nanovesicles (CDNs) have recently been produced and proposed as exosome-mimetics. Various methods for producing exosome-mimetics have been developed. In this chapter, we present a simple, efficient, and cost-effective CDNs production method that uses common laboratory equipment (microcentrifuge) and spin cups. Through a series of extrusion and size exclusion steps, CDNs are produced from in vitro cell culture and are found to highly resemble the endogenous exosomes. Thus, we envision that this strategy holds great potential as a viable alternative to exosomes in the development of ideal DDS.
细胞衍生的药物递送系统（DDS），特别是外泌体，由于其内在的靶向能力，越来越受到关注，并被越来越多地探索作为新型 DDS。然而，外泌体的临床转化受到繁琐的分离程序和低产量的阻碍。细胞衍生的纳米囊泡（CDNs）最近已经被生产出来，并被提议作为外泌体模拟物。已经开发了各种生产外泌体模拟物的方法。在本章中，我们提出了一种简单、高效和具有成本效益的 CDNs 生产方法，该方法使用常见的实验室设备（微量离心机）和旋转杯。通过一系列挤压和大小排除步骤，从体外细胞培养中产生 CDNs，并且发现它们非常类似于内源性外泌体。因此，我们设想，这种策略作为理想 DDS 中替代外泌体的可行方法具有很大的潜力。
