Secondary Department of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang, China.
Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12216-12223. doi: 10.26355/eurrev_202012_24012.
This study aims to uncover the differential expression of circRNA_100395 in breast carcinoma specimens, and its regulatory effect on cancer cell phenotypes. The role of circRNA_100395 in affecting breast carcinoma progression and the molecular mechanism are explored as well.
CircRNA_100395 expressions in breast carcinoma and paracancerous tissues were detected. The influence of circRNA_100395 level on clinical indicators of breast carcinoma patients was analyzed. In vitro regulations of circRNA_100395 on phenotypes of breast carcinoma cells were examined by CCK-8, colony formation, and transwell assay. The interaction between circRNA_100395 and MAPK6 was confirmed by Dual-Luciferase reporter assay and rescue assays.
CircRNA_100395 was downregulated in breast carcinoma tissues and cell lines. Its level was negatively correlated to tumor staging and tumor size of breast carcinoma. Overexpression of circRNA_100395 in SKBR3 and MDA-MB-231 cells weakened proliferative and migratory abilities. MAPK6 was the target gene of circRNA_100395. Overexpression of MAPK6 reversed the anti-cancer effect of circRNA_100395 on breast carcinoma.
CircRNA_100395 serves as an anti-cancer gene in breast carcinoma progression by targeting MAPK6, and its level is negatively correlated to tumor staging and tumor size of breast carcinoma. CircRNA_100395 can be utilized as a potential biomarker and therapeutic target of breast carcinoma.
本研究旨在揭示 circRNA_100395 在乳腺癌标本中的差异表达及其对癌细胞表型的调控作用。还探讨了 circRNA_100395 影响乳腺癌进展的作用及其分子机制。
检测乳腺癌及癌旁组织中 circRNA_100395 的表达，分析 circRNA_100395 水平对乳腺癌患者临床指标的影响。通过 CCK-8、集落形成和 Transwell 实验检测 circRNA_100395 对乳腺癌细胞表型的体外调控作用。通过双荧光素酶报告实验和挽救实验证实 circRNA_100395 与 MAPK6 之间的相互作用。
circRNA_100395 在乳腺癌组织和细胞系中下调。其水平与乳腺癌的肿瘤分期和肿瘤大小呈负相关。在 SKBR3 和 MDA-MB-231 细胞中过表达 circRNA_100395 可减弱其增殖和迁移能力。MAPK6 是 circRNA_100395 的靶基因。过表达 MAPK6 可逆转 circRNA_100395 对乳腺癌的抗癌作用。
circRNA_100395 通过靶向 MAPK6 在乳腺癌进展中发挥抑癌基因的作用，其水平与乳腺癌的肿瘤分期和肿瘤大小呈负相关。circRNA_100395 可作为乳腺癌的潜在生物标志物和治疗靶点。
