Department of Cell Biology & Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
UNC Kidney Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Biotechniques. 2021 Mar;70(3):181-185. doi: 10.2144/btn-2020-0152. Epub 2020 Dec 18.
Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods.
血管在健康和疾病中都起着至关重要的作用。了解血管的形成、模式和对损伤的反应是至关重要的。然而，标记和成像血管以确定这些特性可能是困难和耗时的。在这里，作者提出了一种新的方法，通过利用 Evans 蓝的荧光特性，快速有效地标记整个血管网络。通过将标记与荧光显微镜结合使用，与传统方法相比，这种方法可以在更短的时间和更低的成本下实现整个组织血管的可视化。
