Kozler P, Pokorný J
Department of Neurosurgery, Central Military Hospital, First Faculty of Medicine, Charles University, U vojenské nemocnice 1200, 169 02 Prague 6, Czech Republic.
Sb Lek. 2003;104(3):255-62.
Evans blue was applied to 12 rats by way of intracarotid injection into the common carotid artery or internal carotid artery both with the blood-brain barrier intact and after its mannitol-induced osmotic opening. For each type of application, a histological picture of Evans blue propagation through the brain was obtained by means of fluorescence microscopy. An assessment was made of the overall intensity of staining, and the ratio was established of the intra:extra-cellular Evans blue distribution in the cortex and hippocampus of both hemispheres. The histological picture obtained on injecting Evans blue into the internal carotic artery two minutes after the blood-brain barrier opening with mannitol can be seen as morphological evidence of the fact that the substance thus applied does have an effect on cell homeostasis since the intracellular share of Evans blue distribution is considerable.
将伊文思蓝通过颈总动脉或颈内动脉进行颈内注射，应用于12只大鼠，其中一部分血脑屏障完整，另一部分在甘露醇诱导渗透性开放血脑屏障后进行注射。对于每种注射方式，通过荧光显微镜获得伊文思蓝在脑内扩散的组织学图像。评估染色的总体强度，并确定双侧大脑皮质和海马中细胞内与细胞外伊文思蓝分布的比例。在用甘露醇开放血脑屏障两分钟后将伊文思蓝注入颈内动脉所获得的组织学图像，可以被视为这样一个事实的形态学证据，即如此应用的物质确实对细胞稳态有影响，因为伊文思蓝分布的细胞内部分相当可观。
