Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Department of Pathology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
Emerg Microbes Infect. 2021 Dec;10(1):81-96. doi: 10.1080/22221751.2020.1866955.
species are wood-inhabiting fungi and emerging opportunistic pathogens causing phaeohyphomycosis. In this study, we isolated a dematiaceous fungus, HKU44, from the subhepatic abscess pus and drain fluids of a liver transplant recipient with post-transplant biliary and hepatico-jejunostomy bypass strictures. Histology of the abscess wall biopsy showed abundant fungal hyphae. The patient survived after a second liver transplant and antifungal therapy. On SDA, HKU44 grew initially as white powdery colonies which turned beige upon maturation. Hyphae were septate and hyaline. Phialides were monophialidic and laterally located, generally closely associated to a cluster of conidia which were usually reniform. Phylogenetic analyses showed that HKU44 is most closely related to, but distinct from, and . These suggested that HKU44 is a novel species, for which the name sp. nov. is proposed. Antifungal susceptibility testing showed that species possessed high MICs/MECs for fluconazole, 5-flucytosine and the echinocandins; whereas they exhibited a high strain-to-strain variability to the susceptibilities to the other triazoles. As for amphotericin B, ∼65% of the strains had low MICs (≤1 µg/mL). DNA sequencing should be performed to accurately identify fungi with -like morphologies, so is antifungal susceptibility testing for patients with infections.
种是寄生于木材的真菌和新兴的机会性病原体，引起暗色丝孢霉病。在本研究中，我们从肝移植受者的肝下脓肿脓液和引流液中分离出一株暗色真菌，该受者患有移植后胆道和肝肠吻合口狭窄。脓肿壁活检的组织学显示有大量真菌菌丝。在进行第二次肝移植和抗真菌治疗后，患者存活下来。在 SDA 上，HKU44 最初生长为白色粉状菌落，成熟后变为米色。菌丝有隔膜，呈透明状。瓶梗单枝，侧向生，通常与一群通常呈肾形的分生孢子紧密相关。系统发育分析表明，HKU44 与 和 最为密切相关，但与它们不同。这表明 HKU44 是一种新型 的物种，建议将其命名为 sp. nov.。抗真菌药敏试验表明， 种对氟康唑、5-氟胞嘧啶和棘白菌素类药物的 MIC/MEC 值较高；而它们对其他三唑类药物的敏感性存在较高的菌株间变异性。对于两性霉素 B，约 65%的 菌株具有较低的 MIC（≤1μg/mL）。应进行 DNA 测序以准确鉴定具有 -样形态的真菌，因此对于 感染患者应进行抗真菌药敏试验。
