Molinari A, Formisano G, Malorni W
Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
Cell Biol Toxicol. 1987 Dec;3(4):417-30. doi: 10.1007/BF00119914.
Class I histocompatibility antigens (HLA) are expressed on the surface of almost all nucleated mammalian cells; the expression of this surface antigenic molecule may be changed or abrogated by several factors. In this paper, a modification in HLA expression in a human carcinoma cell line following exposure to the neurotoxicant 2,5 hexanedione is reported. This compound is known to produce a wide spectrum of subcellular pathological events; in this study, we describe an effect on the surface and cytoplasmic distribution of both light and heavy subunits of HLA antigens, demonstrated by immunocytochemical and immunoelectron microscopy techniques. Human carcinoma cells, which under normal growing conditions express the HLA, abrogate the surface expression of this glycoprotein after exposure to 2,5 hexanedione and an intracytoplasmic accumulation seems to occur. Several possibilities are discussed, such as an effect of the toxicant on the transport of the nascent glycoprotein.
I类组织相容性抗原（HLA）在几乎所有有核哺乳动物细胞表面表达；该表面抗原分子的表达可能会因多种因素而改变或消除。本文报道了人癌细胞系在暴露于神经毒剂2,5 -己二酮后HLA表达的改变。已知该化合物会引发广泛的亚细胞病理事件；在本研究中，我们通过免疫细胞化学和免疫电子显微镜技术描述了其对HLA抗原轻、重亚基的表面及胞质分布的影响。在正常生长条件下表达HLA的人癌细胞，在暴露于2,5 -己二酮后会消除这种糖蛋白的表面表达，且似乎会发生胞质内积累。文中讨论了几种可能性，比如该毒物对新生糖蛋白转运的影响。
