Clin Lab. 2020 Dec 1;66(12). doi: 10.7754/Clin.Lab.2020.191265.
Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide. Based on clinical data, CRC could be cured by surgery with favorable outcomes if diagnosed at an early stage. The present study aimed to determine whether circ-FMN2, circ-LMNB1, and circ-ZNF609 may serve as potential biomarkers for CRC.
Expression levels of circ-FMN2, circ-LMNB1, and circ-ZNF609 were detected in serum samples from 88 CRC patients and 68 healthy volunteers by real-time quantitative PCR (RT-qPCR). The correlation between circRNA expressions and clinicopathological parameters was analyzed subsequently. The ROC curve analysis and survival curves were calculated and compared in order to explore the diagnostic and prognostic values of circ-RNAs in CRC.
The results verified that circ-FMN2, circ-LMNB1, and circ-ZNF609 were significantly elevated in serum samples of CRC patients compared with healthy controls (p < 0.01). Increased circ-FMN2, circ-LMNB1, and circ-ZNF609 expressions were markedly positively correlated with histological grade (p < 0.0001, p = 0.0014, p = 0.0303), lymph nodes metastasis (p < 0.0001, p < 0.0001, p = 0.0093), and TNM stage (p = 0.0055, p = 0.0110, p < 0.000). Meanwhile, the ROC curve analysis verified the diagnostic accuracy of circ-FMN2, circ-LMNB1, and circ-ZNF609 with AUC of 0.9153 (95% CI = 0.8707 ~ 0.9599), 0.9627 (95% CI = 0.9351 ~ 0.9903), and 0.8711 (95% CI = 0.8151 ~ 0.9270), respectively. Furthermore, the CRC patients with high circ-FMN2, circ-LMNB1, and circ-ZNF609 had significantly worse outcomes than those with low expression (p = 0.0267, p = 0.0145, p = 0.0194).
The present study elucidated that circ-FMN2, circ-LMNB1, and circ-ZNF609 may function as potential diagnostic and prognostic indicators for CRC detection.
结直肠癌（CRC）是全球最常见的癌症之一。基于临床数据，如果在早期诊断，CRC 可以通过手术治愈，预后良好。本研究旨在确定 circ-FMN2、circ-LMNB1 和 circ-ZNF609 是否可作为 CRC 的潜在生物标志物。
通过实时定量 PCR（RT-qPCR）检测 88 例 CRC 患者和 68 例健康志愿者血清样本中的 circ-FMN2、circ-LMNB1 和 circ-ZNF609 的表达水平。随后分析 circRNA 表达与临床病理参数之间的相关性。计算并比较 ROC 曲线分析和生存曲线，以探讨 circRNA 在 CRC 中的诊断和预后价值。
结果证实，与健康对照组相比，CRC 患者的血清样本中 circ-FMN2、circ-LMNB1 和 circ-ZNF609 显著升高（p<0.01）。circ-FMN2、circ-LMNB1 和 circ-ZNF609 的表达增加与组织学分级（p<0.0001、p=0.0014、p=0.0303）、淋巴结转移（p<0.0001、p<0.0001、p=0.0093）和 TNM 分期（p=0.0055、p=0.0110、p<0.000）明显正相关。同时，ROC 曲线分析验证了 circ-FMN2、circ-LMNB1 和 circ-ZNF609 的诊断准确性，AUC 分别为 0.9153（95%CI=0.87070.9599）、0.9627（95%CI=0.93510.9903）和 0.8711（95%CI=0.8151~0.9270）。此外，circ-FMN2、circ-LMNB1 和 circ-ZNF609 高表达的 CRC 患者的结局明显比低表达的患者差（p=0.0267、p=0.0145、p=0.0194）。
本研究表明，circ-FMN2、circ-LMNB1 和 circ-ZNF609 可能作为 CRC 检测的潜在诊断和预后指标。
