Molecular Medicine Program, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Mol Cell. 2020 Dec 17;80(6):938-939. doi: 10.1016/j.molcel.2020.11.043.
The goal of structural biology is to understand biological macromolecules in as much detail as possible. Depending on the resolution of the structure obtained, insights will range from understanding interactions at the level of proteins, domains, or atoms. The three mainstay structural biology techniques are X-ray crystallography, nuclear magnetic resonance (NMR) imaging, and cryogenic electron microscopy (cryo-EM). Cryo-EM has rapidly gained popularity in recent years due to a combination of hardware and software advances, leading to the so-called Resolution Revolution (Kühlbrandt, 2014).
结构生物学的目标是尽可能详细地了解生物大分子。根据获得的结构分辨率，研究结果将涵盖从蛋白质、结构域或原子水平的相互作用的理解。三种主要的结构生物学技术是 X 射线晶体学、核磁共振（NMR）成像和低温电子显微镜（cryo-EM）。由于硬件和软件的进步，cryo-EM 在近年来迅速流行起来，导致了所谓的分辨率革命（Kühlbrandt，2014）。
