组织培养中细胞类型和细胞密度与含核苷Q的tRNA（tRNATyr、tRNAHis、tRNAAsn和tRNAAsp）同工受体谱的关系
Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATyr, tRNAHis, tRNAAsn and tRNAAsp.
作者信息
Katze J R
出版信息
Nucleic Acids Res. 1978 Jul;5(7):2513-24. doi: 10.1093/nar/5.7.2513.
Abstract
An examination, using reversed-phase chromatography and cyanogen bromide treatment, of tRNATyr, tRNAHis, tRNAAsn, and tRNAAsp from SV40-transformed mouse fibroblasts grown to different cell densities, untransformed cells grown to confluence, and mouse liver indicates that: (1) The tissue cultured mouse fibroblasts examined here are hypomodified with respect to nucleoside Q, while liver tRNA is almost completely modified with respect to Q. (2) Cell density and/or proliferative state do not present as major variables in controlling the expression of Q in the present system. (3) SV40 virus transformation is not a major variable controlling the expression of Q in the present system. The present results support previous use of cyanogen bromide effected shifts in chromatographic elution as an assay for nucleoside Q.
摘要
利用反相色谱法和溴化氰处理，对来自生长至不同细胞密度的SV40转化小鼠成纤维细胞、生长至汇合状态的未转化细胞以及小鼠肝脏的tRNATyr、tRNAHis、tRNAAsn和tRNAAsp进行检测，结果表明：(1) 此处检测的组织培养小鼠成纤维细胞在核苷Q方面修饰不足，而肝脏tRNA在Q方面几乎完全修饰。(2) 在本系统中，细胞密度和/或增殖状态并非控制Q表达的主要变量。(3) 在本系统中，SV40病毒转化不是控制Q表达的主要变量。本研究结果支持先前使用溴化氰引起的色谱洗脱变化作为核苷Q的检测方法。
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Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATyr, tRNAHis, tRNAAsn and tRNAAsp.组织培养中细胞类型和细胞密度与含核苷Q的tRNA（tRNATyr、tRNAHis、tRNAAsn和tRNAAsp）同工受体谱的关系
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